{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:38:01Z","timestamp":1784191081919,"version":"3.55.0"},"reference-count":6,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n We describe the evaluation of culture-negative synovial fluid from a 3-year-old boy by PCR and electrospray ionization followed by mass spectrometry (PCR\/ESI-MS). Our patient developed a diffuse rash and fever with systemic signs and symptoms of sepsis, but four sets of blood cultures obtained prior to initiation of antibiotics were negative. After 1 week of illness, he developed right-knee swelling. Analysis of synovial fluid was consistent with infection, but cultures of specimens obtained following initiation of antimicrobial treatment were negative for growth. PCR\/ESI-MS detected\n Streptobacillus moniliformis<\/jats:named-content>\n in the synovial fluid sample. Our patient completed an appropriate course of antibiotic treatment and remained completely asymptomatic in follow-up evaluation. This unique case suggests that PCR\/ESI-MS may be a useful diagnostic tool for direct detection of unusual or unexpected pathogens directly from clinical specimens, particularly when samples have been obtained from patients following initiation of antibiotic therapy.\n <\/jats:p>","DOI":"10.1128\/jcm.00259-14","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T03:55:23Z","timestamp":1397102123000},"page":"2259-2261","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Direct Detection of Indirect Transmission of Streptobacillus moniliformis Rat Bite Fever Infection"],"prefix":"10.1128","volume":"52","author":[{"given":"Joseph R.","family":"Mackey","sequence":"first","affiliation":[{"name":"Department of Pediatrics, St. Francis Medical Center, Peoria, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsa L. Vazquez","family":"Melendez","sequence":"additional","affiliation":[{"name":"University of Illinois School of Medicine, Department of Medicine and Pediatrics, Peoria, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Farrell","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Disease, University of Illinois College of Medicine, Peoria, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin S.","family":"Lowery","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, an Abbott Company, Carlsbad, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan A.","family":"Rounds","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, an Abbott Company, Carlsbad, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rangarajan","family":"Sampath","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, an Abbott Company, Carlsbad, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Research Service, Cleveland, Ohio, USA"},{"name":"Case Western Reserve University, Departments of Medicine, Pharmacology and Molecular Microbiology, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400516"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02305-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066349"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00936-10"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00016-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00432-14"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00259-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00259-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:58:25Z","timestamp":1646481505000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00259-14"}},"subtitle":[],"editor":[{"given":"R.","family":"Patel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":6,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1128\/JCM.00259-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00259-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]},"assertion":[{"value":"2014-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}