{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:10:35Z","timestamp":1788959435030,"version":"build-2803163510"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"German Federal Ministry of Education and Research","award":["16GW0149K"],"award-info":[{"award-number":["16GW0149K"]}]},{"name":"German Federal Ministry of Education and Research","award":["16GW0150"],"award-info":[{"award-number":["16GW0150"]}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["GHS-20-0010"],"award-info":[{"award-number":["GHS-20-0010"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The rise of multidrug-resistant microorganisms highlights the need for rapid antimicrobial susceptibility testing (AST) to guide early and effective therapy. This study evaluated a novel MALDI-TOF MS-based assay (MBT FAST, Bruker, Germany), which employs the direct-on-target microdroplet growth assay (DOT-MGA) for phenotypic AST. A total of 243\n                      <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                      isolates representing diverse species and resistance patterns were tested against a broad panel of antibiotics. Each isolate was incubated in 6-\u00b5L microdroplets of cation-adjusted Mueller-Hinton broth, with and without antibiotics at two-fold serial dilutions, at 35\u00b0C for 6 h inside a humidity-controlled chamber (MBT FAST Shuttle, Bruker). Broth was removed using a prototype device (MBT FAST Stamp, Bruker), and MALDI-TOF MS measurements were performed with the MALDI Biotyper Sirius system (Bruker). Results were compared with broth microdilution as the standard for determining minimum inhibitory concentrations (MIC). Valid growth control was achieved for 100% of isolates. Essential agreement (EA), defined as MICs within \u00b11 two-fold dilution step of the standard method, was as follows: amoxicillin\/clavulanic acid 96.3%, amikacin 97.5%, aztreonam 95.5%, ceftazidime\/avibactam 95.5%, ceftazidime 94.6%, cefepime 92.2%, ciprofloxacin 91.8%, colistin 92.2%, ceftriaxone 95.5%, cefotaxime 94.2%, gentamicin 95.5%, imipenem 91.8%, levofloxacin 97.9%, meropenem 87.2%, meropenem\/vaborbactam 94.2%, piperacillin\/tazobactam 90.9%, trimethoprim\/sulfamethoxazole 90.9%, temocillin 93.3%, and tobramycin 97.5%. The overall EA across all antimicrobials tested reached 96.0%. The research-use-only MBT FAST assay provides reliable AST within 6 h, substantially reducing turnaround time. Building on technology widely implemented in diagnostic laboratories, this approach holds promise for integration into microbiology practice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Standard antimicrobial susceptibility testing requires prolonged incubation, delaying targeted treatment and de-escalation. This study evaluated MBT FAST, a MALDI-TOF MS-based assay that measures bacterial growth directly on the MALDI target plate in the presence of antibiotics. In a diverse collection of\n                      <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                      tested against a panel of 19 antibiotics, the assay provided reliable minimum inhibitory concentration results after 6 h and showed high agreement with standard broth microdilution. Because MALDI-TOF MS instruments are widely used in clinical microbiology laboratories, this approach could make rapid phenotypic susceptibility testing easier to integrate into routine workflows.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00259-26","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:00:38Z","timestamp":1787310038000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Rapid MALDI-TOF MS antimicrobial susceptibility testing with the MBT FAST assay"],"prefix":"10.1128","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4207-5290","authenticated-orcid":true,"given":"Evgeny A.","family":"Idelevich","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/025vngs54","id-type":"ROR","asserted-by":"publisher"}],"name":"Friedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald","place":["Greifswald, Germany"]},{"id":[{"id":"https:\/\/ror.org\/01856cw59","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Medical Microbiology, University Hospital M\u00fcnster","place":["M\u00fcnster, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Alpers","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Buhl","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03z3mg085","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Clinical Microbiology, Infectious Diseases and Infection Control, Paracelsus Medical University, Klinikum N\u00fcrnberg","place":["N\u00fcrnberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Drews","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen P.","family":"Kidd","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01bbyhp53","id-type":"ROR","asserted-by":"publisher"}],"name":"Basingstoke and North Hampshire Hospital, Infection Sciences Department, Hampshire Hospitals NHS Foundation Trust","place":["Basingstoke, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Kostrzewa","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Moore","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01bbyhp53","id-type":"ROR","asserted-by":"publisher"}],"name":"Basingstoke and North Hampshire Hospital, Infection Sciences Department, Hampshire Hospitals NHS Foundation Trust","place":["Basingstoke, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilka D.","family":"Nix","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Oberheitmann","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatima","family":"Pereira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01bbyhp53","id-type":"ROR","asserted-by":"publisher"}],"name":"Basingstoke and North Hampshire Hospital, Infection Sciences Department, Hampshire Hospitals NHS Foundation Trust","place":["Basingstoke, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Pranada","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology, MVZ Dr. Eberhard & Partner Dortmund","place":["Dortmund, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Scholz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04rcqnp59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Heidelberg University Hospital","place":["Heidelberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7982-3430","authenticated-orcid":true,"given":"Christian","family":"Walter","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04rcqnp59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Heidelberg University Hospital","place":["Heidelberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Sparbier","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Sp\u00fcler","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Steinmann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03z3mg085","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Clinical Microbiology, Infectious Diseases and Infection Control, Paracelsus Medical University, Klinikum N\u00fcrnberg","place":["N\u00fcrnberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline","family":"Weferling","sequence":"additional","affiliation":[{"name":"Bruker Daltonics GmbH & Co. KG","place":["Bremen, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5601-7934","authenticated-orcid":true,"given":"Stefan","family":"Zimmermann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04rcqnp59","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Heidelberg University Hospital","place":["Heidelberg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6391-1341","authenticated-orcid":true,"given":"Karsten","family":"Becker","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/025vngs54","id-type":"ROR","asserted-by":"publisher"}],"name":"Friedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald","place":["Greifswald, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/23.11_ts.1168"},{"key":"e_1_3_4_3_2","first-page":"382","article-title":"A new method for the rapid determination of bacterial sensitivity","volume":"5","author":"Rogers MA","year":"1955","unstructured":"Rogers MA, Ryan WL, Severens JM. 1955. A new method for the rapid determination of bacterial sensitivity. Antibiot Chemother (Northfield) 5:382\u2013385.","journal-title":"Antibiot Chemother (Northfield)"},{"key":"e_1_3_4_4_2","first-page":"351","article-title":"A new rapid plate method for determining antibiotic sensitivity","volume":"6","author":"Pital A","year":"1956","unstructured":"Pital A, Disque DT, Leise JM. 1956. A new rapid plate method for determining antibiotic sensitivity. Antibiot Chemother (Northfield) 6:351\u2013359.","journal-title":"Antibiot Chemother (Northfield)"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0098-9"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30477-1"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-0087"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-015-0795-y"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac727"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.04.025"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53930-x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.03.024"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.11.017"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanepe.2025.101570"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01744"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01814-19"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.10.016"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00913-18"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00232"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00013"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61890-7"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2019-0106"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2020.8976"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S303187"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2020.115093"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.12025\/j.issn.1008-6358.2020.20201138"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1075650"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S356045"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S336987"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-025-01833-4"},{"key":"e_1_3_4_31_2","unstructured":"ISO. 2021. 20776-2. Clinical laboratory testing and in vitro diagnostic test systems - Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices - Part 2: evaluation of performance of antimicrobial susceptibility test devices against reference broth microdilution. Second edition. International Organization for Standardization. Geneva Switzerland"},{"key":"e_1_3_4_32_2","unstructured":"ISO. 2019. 20776-1. Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices - Part 1: broth micro-dilution reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. Second edition. International Organization for Standardization. Geneva Switzerland"},{"key":"e_1_3_4_33_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard","author":"CLSI","year":"2024","unstructured":"CLSI. 2024. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 12th ed. Clinical Laboratory Standards Institute, Wayne, PA.","edition":"12"},{"key":"e_1_3_4_34_2","unstructured":"FDA. 2009. Guidance for industry and FDA - class II special controls guidance document: antimicrobial susceptibility test (AST) systems. US Food and Drug Administration. Silver Spring MD"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2018.05.003"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofv075"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00259-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00259-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:01:20Z","timestamp":1788958880000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00259-26"}},"subtitle":[],"editor":[{"given":"Erin","family":"McElvania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,9]]},"references-count":35,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,9]]}},"alternative-id":["10.1128\/jcm.00259-26"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00259-26","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,9]]},"assertion":[{"value":"2026-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00259-26"}}