{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T05:34:19Z","timestamp":1790573659597,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Nocardiosis is a rare disease that is caused by Gram-positive actinobacteria of the\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            genus and affects predominantly immunocompromised patients. In its disseminated form, it has a predilection for the central nervous system and is associated with high mortality rates. Therefore, prompt identification of the pathogen is critical. Matrix-assisted laser desorption ionization\u2013time of flight (MALDI-TOF) mass spectrometry is a relatively novel technique used for identification of microorganisms. In this work, an upgraded MALDI-TOF Biotyper database containing\n            <jats:named-content content-type=\"genus-species\">Corynebacterineae<\/jats:named-content>\n            representatives of strains deposited in the Polish Collection of Microorganisms was created and used for identification of the strain isolated from a nocardial brain abscess, mimicking a brain tumor, in an immunocompetent patient. Testing with the API Coryne system initially incorrectly identified\n            <jats:named-content content-type=\"genus-species\">Rhodococcus<\/jats:named-content>\n            sp., while chemotaxonomic tests, especially mycolic acid analysis, enabled correct\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            identification only at the genus level. Subsequent sequence analysis of 16S rRNA and\n            <jats:italic>secA1<\/jats:italic>\n            genes confirmed the identification. To improve the accuracy of the results, an in-house database was constructed using optimized parameters; with the use of the database, the strain was eventually identified as\n            <jats:named-content content-type=\"genus-species\">Nocardia farcinica<\/jats:named-content>\n            . Clinical laboratories processing various clinical strains can upgrade a commercial database to improve and to accelerate the results obtained. This is especially important in the case of\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            , for which valid microbial diagnosis remains challenging; reference laboratories are often required to identify and to survey these rare actinobacteria.\n          <\/jats:p>","DOI":"10.1128\/jcm.00268-15","type":"journal-article","created":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T11:33:08Z","timestamp":1433417588000},"page":"2611-2621","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Creation of an In-House Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates"],"prefix":"10.1128","volume":"53","author":[{"given":"Mariola","family":"Pa\u015bciak","sequence":"first","affiliation":[{"name":"Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W\u0142adys\u0142aw","family":"Dacko","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Regional Specialist Hospital, Legnica, Poland"},{"name":"Department of Neurosurgery, Specialist Medical Center, Polanica-Zdr\u00f3j, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Sikora","sequence":"additional","affiliation":[{"name":"Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danuta","family":"Gurlaga","sequence":"additional","affiliation":[{"name":"Department of Microbial Diagnostics, Regional Specialist Hospital, Legnica, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Pawlik","sequence":"additional","affiliation":[{"name":"Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"Mi\u0119kisiak","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Specialist Medical Center, Polanica-Zdr\u00f3j, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Gamian","sequence":"additional","affiliation":[{"name":"Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroc\u0142aw, Poland"},{"name":"Wroc\u0142aw Research Center EIT+, Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.2.213"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/600885"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03311.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01881-09"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2011.02833.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.3630080106"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02135-10"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024720"},{"key":"e_1_3_3_10_2","volume-title":"Document M24-A2","author":"Clinical and Laboratory Standards Institute","year":"2011","unstructured":"Clinical and Laboratory Standards Institute. 2011. 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