{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:18:41Z","timestamp":1790144321427,"version":"4.0.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["Appropriated Funds"],"award-info":[{"award-number":["Appropriated Funds"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n            is the causative agent of plague and is considered a category A priority pathogen due to its potential for high transmissibility and the significant morbidity and mortality it causes in humans.\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            is endemic to the western United States and much of the world, necessitating programs to monitor for this pathogen on the landscape. Elevated human risk of plague infection has been spatially correlated with spikes in seropositive wildlife numbers, particularly rodent-eating carnivores, which are frequently in contact with the enzootic hosts and the associated arthropod vectors of\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            . In this study, we describe a semiautomated bead-based flow cytometric assay developed for plague monitoring in wildlife called the F1 Luminex plague assay (F1-LPA). Based upon Luminex\/Bio-Plex technology, the F1-LPA targets serological responses to the F1 capsular antigen of\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            and was optimized to analyze antibodies eluted from wildlife blood samples preserved on Nobuto filter paper strips. In comparative evaluations with passive hemagglutination, the gold standard tool for wildlife plague serodiagnosis, the F1-LPA demonstrated as much as 64\u00d7 improvement in analytical sensitivity for F1-specific IgG detection and allowed for unambiguous classification of IgG status. The functionality of the F1-LPA was demonstrated for coyotes and other canids, which are the primary sentinels in wildlife plague monitoring, as well as felids and raccoons. Additionally, assay formats that do not require species-specific immunological reagents, which are not routinely available for several wildlife species used in plague monitoring, were determined to be functional in the F1-LPA.\n          <\/jats:p>","DOI":"10.1128\/jcm.00273-18","type":"journal-article","created":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T09:18:07Z","timestamp":1524561487000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["A Bead-Based Flow Cytometric Assay for Monitoring Yersinia pestis Exposure in Wildlife"],"prefix":"10.1128","volume":"56","author":[{"given":"Jeffrey C.","family":"Chandler","sequence":"first","affiliation":[{"name":"National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie A.","family":"Baeten","sequence":"additional","affiliation":[{"name":"National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, Colorado, USA"},{"name":"Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doreen L.","family":"Griffin","sequence":"additional","affiliation":[{"name":"National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Gidlewski","sequence":"additional","affiliation":[{"name":"National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"DeLiberto","sequence":"additional","affiliation":[{"name":"National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeannine M.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Pappert","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Young","sequence":"additional","affiliation":[{"name":"Division of Vector-Borne Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah N.","family":"Bevins","sequence":"additional","affiliation":[{"name":"National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, Fort Collins, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2008039"},{"key":"e_1_3_3_3_2","first-page":"237","article-title":"Surveillance and control of bubonic plague in the United States","volume":"50","author":"Barnes AM","year":"1982","unstructured":"Barnes AM. 1982. Surveillance and control of bubonic plague in the United States. Symp Zool Soc (London) 50:237\u2013270.","journal-title":"Symp Zool Soc (London)"},{"key":"e_1_3_3_4_2","volume-title":"Plague manual: epidemiology, distribution, surveillance and control","author":"Gage KL","year":"1999","unstructured":"Gage KL. 1999. Plague surveillance. In Plague manual: epidemiology, distribution, surveillance and control. World Health Organization, Geneva, Switzerland. http:\/\/www.who.int\/csr\/resources\/publications\/plague\/WHO_CDS_CSR_EDC_99_2_EN\/en\/. Accessed 7 September 2017."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1948-7134.2009.00004.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2010.0196"},{"key":"e_1_3_3_7_2","first-page":"527","article-title":"The role of domestic animals in the epidemiology of plague. 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