{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T18:33:37Z","timestamp":1780598017299,"version":"3.54.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Smallpox, once a devastating disease caused by\n            <jats:italic>Variola virus<\/jats:italic>\n            , a member of the\n            <jats:italic>Orthopoxvirus<\/jats:italic>\n            genus, was eradicated in 1980. However, the importance of variola virus infections has been stressed widely in the last few years, particularly following recent social events in the world. Today, variola virus is considered to be one of the most significant agents with potential use as a biological weapon. In this study we developed an internally controlled real-time PCR assay for rapid detection and simultaneous differentiation of variola virus from other orthopoxviruses. The assay is based on TaqMan 3\u2032-minor groove binder (MGB) chemistry and uses generic primers, designed in highly conserved genomic regions of the\n            <jats:italic>crmB<\/jats:italic>\n            gene, and three TaqMan MGB probes designed to identify orthopoxviruses, variola virus, and an internal control. The results obtained suggest that the assay is rapid, sensitive, specific, and suitable for the generic detection of orthopoxviruses and the identification of variola virus and avoids false-negative results in a single reaction tube.\n          <\/jats:p>","DOI":"10.1128\/jcm.00276-06","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T18:54:05Z","timestamp":1165431245000},"page":"4464-4470","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Use of Internally Controlled Real-Time Genome Amplification for Detection of Variola Virus and Other Orthopoxviruses Infecting Humans"],"prefix":"10.1128","volume":"44","author":[{"given":"C. G.","family":"Fedele","sequence":"first","affiliation":[{"name":"Alerts and Emergencies Unit, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"},{"name":"Arbovirus and Imported Viral Diseases Laboratory, Diagnostic Microbiology Service, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Negredo","sequence":"additional","affiliation":[{"name":"Arbovirus and Imported Viral Diseases Laboratory, Diagnostic Microbiology Service, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Molero","sequence":"additional","affiliation":[{"name":"Arbovirus and Imported Viral Diseases Laboratory, Diagnostic Microbiology Service, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. P.","family":"Sa\u0301nchez-Seco","sequence":"additional","affiliation":[{"name":"Alerts and Emergencies Unit, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"},{"name":"Arbovirus and Imported Viral Diseases Laboratory, Diagnostic Microbiology Service, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tenorio","sequence":"additional","affiliation":[{"name":"Arbovirus and Imported Viral Diseases Laboratory, Diagnostic Microbiology Service, National Center of Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.13.2657"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(98)80121-5"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(94)00173-E"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(97)00035-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.1985-1988.2002"},{"key":"e_1_3_2_7_2","first-page":"986","volume":"10","year":"1996","unstructured":"Heid, C. 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