{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T02:26:28Z","timestamp":1778811988432,"version":"3.51.4"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            KPC-type carbapenemases are emerging in\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioM\u00e9rieux EasyQ real-time PCR assay for\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            detection with 300 members of the\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            , including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>KPC<\/jats:sub>\n            genes, with no false positives, and results were available within 2 h.\n          <\/jats:p>","DOI":"10.1128\/jcm.00284-12","type":"journal-article","created":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T04:20:29Z","timestamp":1337833229000},"page":"2783-2785","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Evaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of Klebsiella pneumoniae Carbapenemase Genes (\n            <i>bla<\/i>\n            <sub>KPC<\/sub>\n            )"],"prefix":"10.1128","volume":"50","author":[{"given":"Teresa","family":"Spanu","sequence":"first","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Fiori","sequence":"additional","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziana","family":"D'Inzeo","sequence":"additional","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Canu","sequence":"additional","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serena","family":"Campoli","sequence":"additional","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tommaso","family":"Giani","sequence":"additional","affiliation":[{"name":"Section of Microbiology, Department of Biotechnologies, University of Siena"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Palucci","sequence":"additional","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Tumbarello","sequence":"additional","affiliation":[{"name":"Infectious Diseases, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Sanguinetti","sequence":"additional","affiliation":[{"name":"Institutes of Microbiology, Agostino Gemelli Hospital, Catholic University of the Sacred Heart, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Section of Microbiology, Department of Biotechnologies, University of Siena"},{"name":"Clinical Microbiology and Virology Unit, Siena University Hospital, Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1896-1898.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00353-11"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.776-778.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01588-10"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01550-08"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00568-10"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01773-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03294.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01355-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","unstructured":"GrundmannH. 2010. 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