{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T17:11:41Z","timestamp":1770138701795,"version":"3.49.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Wako Chemicals GmbH"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Currently, diagnosis of\n            <jats:named-content content-type=\"genus-species\">Pneumocystis jirovecii<\/jats:named-content>\n            pneumonia (PJP) relies on analysis of lower respiratory specimens, either by microscopy or quantitative real-time PCR (qPCR). Thus, bronchoscopy is required, which is associated with increased risk of respiratory failure. We assessed the value of noninvasive serologic \u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan (BDG) testing for laboratory diagnosis of PJP using a newly available turbidimetric assay. We identified 73 cases of PJP with positive qPCR results from lower respiratory specimens for\n            <jats:named-content content-type=\"genus-species\">Pneumocystis<\/jats:named-content>\n            and serology samples dating from 1 week before to 4 weeks after qPCR. In addition, 25 sera from controls with suspected PJP but specimens negative for\n            <jats:named-content content-type=\"genus-species\">Pneumocystis<\/jats:named-content>\n            by qPCR were identified. Sera were tested with a turbidimetric BDG assay (Fujifilm Wako Chemicals Europe GmbH, Neuss, Germany), using an 11-pg\/ml cutoff. Sensitivity and specificity were calculated based on qPCR test results as a reference. The turbidimetric BDG assay identified 63\/73 patients with positive or slightly positive qPCR tests for an overall sensitivity of 86%; after exclusion of cases with only slightly positive qPCR results, sensitivity was 91%. No correlation between serum BDG levels and respiratory specimen DNA levels was found. Serologic BDG testing was negative in 25\/25 controls with negative qPCR for a specificity of 100% using the predefined cutoff. In 22\/25 samples (88%), no BDG was detected. Serologic BDG testing using the turbidimetric assay showed high sensitivity and specificity compared to qPCR of lower respiratory specimens for the diagnosis of PJP. Both turnover time and test performance will allow clinicians to delay or in some cases forego bronchoscopy.\n          <\/jats:p>","DOI":"10.1128\/jcm.00286-18","type":"journal-article","created":{"date-parts":[[2018,5,3]],"date-time":"2018-05-03T09:19:03Z","timestamp":1525339143000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of a Turbidimetric \u03b2-\n            <scp>d<\/scp>\n            -Glucan Test for Detection of Pneumocystis jirovecii Pneumonia"],"prefix":"10.1128","volume":"56","author":[{"given":"Karl","family":"Dichtl","sequence":"first","affiliation":[{"name":"Max von Pettenkofer-Institut f\u00fcr Hygiene und Medizinische Mikrobiologie, Medizinische Fakult\u00e4t, LMU M\u00fcnchen, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Seybold","sequence":"additional","affiliation":[{"name":"Sektion Klinische Infektiologie, Medizinische Klinik und Poliklinik IV, Klinikum der Universit\u00e4t, LMU Munich, Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Wagener","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut f\u00fcr Hygiene und Medizinische Mikrobiologie, Medizinische Fakult\u00e4t, LMU M\u00fcnchen, Munich, Germany"},{"name":"Institut f\u00fcr Hygiene und Mikrobiologie, Julius-Maximilians-Universit\u00e4t W\u00fcrzburg, W\u00fcrzburg, Germany"},{"name":"National Reference Center for Invasive Fungal Infections (NRZMyk), Leibniz Institute for Natural Product Research and Infection Biology\u2014Hans Knoell Institute, Jena, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/313843"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/1060028016650107"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw155"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(17)30303-1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw156"},{"key":"e_1_3_2_7_2","unstructured":"Panel on Opportunistic Infections in HIV-Infected Adults and Adolescents. July 2017. Guidelines for the prevention and treatment of opportunistic infections in HIV-infected adults and adolescents: recommendations from the Centers for Disease Control and Prevention the National Institutes of Health and the HIV Medicine Association of the Infectious Diseases Society of America. http:\/\/aidsinfo.nih.gov\/contentfiles\/lvguidelines\/adult_oi.pdf."},{"key":"e_1_3_2_8_2","first-page":"371","article-title":"Utility of galactomannan enzyme immunoassay and (1,3)beta-d-glucan assay in invasive fungal infection","volume":"90","author":"Yu J","year":"2010","unstructured":"Yu J, Li R-Y, Gao L-J, Lu Q-Y, Wang X-H. 2010. Utility of galactomannan enzyme immunoassay and (1,3)beta-d-glucan assay in invasive fungal infection. Zhonghua Yi Xue Za Zhi 90:371\u2013374. 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