{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T02:17:54Z","timestamp":1769739474341,"version":"3.49.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Phoenix system (Becton Dickinson Diagnostic Systems, Sparks, MD) was evaluated for identification (ID) to the species level of streptococci and enterococci. Two hundred clinical isolates were investigated: beta-hemolytic streptococci (\n            <jats:italic>n<\/jats:italic>\n            = 50),\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            organisms (\n            <jats:italic>n<\/jats:italic>\n            = 46), viridans group streptococci (\n            <jats:italic>n<\/jats:italic>\n            = 31),\n            <jats:italic>Enterococcus faecium<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 36),\n            <jats:italic>Enterococcus faecalis<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 25), and other catalase-negative cocci (\n            <jats:italic>n<\/jats:italic>\n            = 12). The API system (bioMe\u0301rieux, Marcy l'E\u0301toile, France) was used as a comparator. Molecular methods (sequencing of 16S rRNA and\n            <jats:italic>zwf<\/jats:italic>\n            and\n            <jats:italic>gki<\/jats:italic>\n            genes and\n            <jats:italic>ddl<\/jats:italic>\n            gene amplification) were used to investigate discordant results. Upon resolution of discrepancies, correct species ID was achieved by the Phoenix system for 121\/129 (93.8%) streptococci and 63\/70 (90.0%) enterococci. Excellent results were obtained for\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            (45\/45) and beta-hemolytic streptococci (49\/50). With regard to viridans streptococci, the accuracy of the Phoenix system was 83.9%. Among the latter organisms, the best performance was obtained with isolates of the\n            <jats:italic>Streptococcus sanguinis<\/jats:italic>\n            group and\n            <jats:italic>Streptococcus anginosus<\/jats:italic>\n            group; problems were instead encountered with the\n            <jats:italic>Streptococcus mitis<\/jats:italic>\n            group. Four\n            <jats:italic>E. faecium<\/jats:italic>\n            and three\n            <jats:italic>E. faecalis<\/jats:italic>\n            isolates were misidentified as\n            <jats:italic>Enterococcus casseliflavus\/Enterococcus gallinarum<\/jats:italic>\n            or\n            <jats:italic>Enterococcus durans<\/jats:italic>\n            . Thus, these isolates were identified only at the genus level. Compared with commercially available systems, the Phoenix system appears a reliable diagnostic tool for identifying clinically relevant streptococci and enterococci. The SMIC\/ID-2 panel proved particularly effective for beta-hemolytic streptococci and pneumococci.\n          <\/jats:p>","DOI":"10.1128\/jcm.00299-06","type":"journal-article","created":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T16:23:28Z","timestamp":1157473408000},"page":"3263-3267","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Use of the Phoenix Automated System for Identification of\n            <i>Streptococcus<\/i>\n            and\n            <i>Enterococcus<\/i>\n            spp"],"prefix":"10.1128","volume":"44","author":[{"given":"Gioconda","family":"Brigante","sequence":"first","affiliation":[{"name":"Laboratory of Medical Microbiology, Ospedale di Circolo and University of Insubria, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Luzzaro","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Ospedale di Circolo and University of Insubria, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessia","family":"Bettaccini","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Ospedale di Circolo and University of Insubria, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianluigi","family":"Lombardi","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Ospedale di Circolo and University of Insubria, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Meacci","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Siena, Siena"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrice","family":"Pini","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Ospedale di Circolo and University of Insubria, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[{"name":"Department of Microbiological Sciences, University of Catania, Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Toniolo","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Microbiology, Ospedale di Circolo and University of Insubria, Varese"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.71-76.2006"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4686-4696.2004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.2065-2073.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5857-5860.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1542-1546.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.24-27.1995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.04.001"},{"key":"e_1_3_2_9_2","first-page":"323","volume":"25","year":"2002","unstructured":"Endimiani, A., F. 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