{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:17:55Z","timestamp":1787807875356,"version":"build-2784847793"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An apparently rare\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            isolate containing one copy of a\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            16S rRNA gene is described herein. This isolate was identified as\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            by biochemical identification methods but generated a positive signal with Gen-Probe Aptima assays for the detection of\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            . Direct 16S rRNA gene sequencing of the purified isolate revealed mixed bases in signature regions that allow for discrimination between\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            . The mixed bases were resolved by sequencing individually PCR-amplified single copies of the genomic 16S rRNA gene. A total of 121 discrete sequences were obtained; 92 (76%) were\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            sequences, and 29 (24%) were\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            sequences. Based on the ratio of species-specific sequences, the\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            strain seems to have replaced one of its four intrinsic 16S rRNA genes with the gonococcal gene. Fluorescence\n            <jats:italic>in situ<\/jats:italic>\n            hybridization (FISH) probes specific for meningococcal and gonococcal rRNA were used to demonstrate the expression of the rRNA genes. Interestingly, the clinical isolate described here expresses both\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            16S rRNA genes, as shown by positive FISH signals with both probes. This explains why the probes for\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            in the Gen-Probe Aptima assays cross-react with this\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            isolate. The\n            <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n            isolate described must have obtained\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -specific DNA through interspecies recombination.\n          <\/jats:p>","DOI":"10.1128\/jcm.00309-13","type":"journal-article","created":{"date-parts":[[2013,7,18]],"date-time":"2013-07-18T05:00:32Z","timestamp":1374123632000},"page":"3199-3206","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Description of an Unusual Neisseria meningitidis Isolate Containing and Expressing Neisseria gonorrhoeae-Specific 16S rRNA Gene Sequences"],"prefix":"10.1128","volume":"51","author":[{"given":"Marion","family":"Walcher","sequence":"first","affiliation":[{"name":"Gen-Probe Incorporated, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhonda","family":"Skvoretz","sequence":"additional","affiliation":[{"name":"Gen-Probe Incorporated, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Montgomery-Fullerton","sequence":"additional","affiliation":[{"name":"Gen-Probe Incorporated, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian","family":"Jonas","sequence":"additional","affiliation":[{"name":"Gen-Probe Incorporated, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Brentano","sequence":"additional","affiliation":[{"name":"Gen-Probe Incorporated, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1996.tb08574.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04964.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01932.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.29261-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3140"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011835"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(00)00168-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026061"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-5-35"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-12-1055"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.031534-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2003.08.006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00057.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-7-314"},{"key":"e_1_3_2_16_2","first-page":"273","article-title":"Global trends in meningococcal disease","volume":"67","author":"Caugant DA","year":"2001","unstructured":"CaugantDA. 2001. 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