{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:57:22Z","timestamp":1788346642557,"version":"build-2803163510"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            To characterize\n            <jats:italic>Shigella<\/jats:italic>\n            clinical strains, we studied 82\n            <jats:italic>Shigella<\/jats:italic>\n            strains recovered from 719 stool samples of patients with bloody diarrhea in Shiraz, Iran, over the period from April to October 2003. Serological assay classified the\n            <jats:italic>Shigella<\/jats:italic>\n            isolates as follows: 61 (74.39%)\n            <jats:italic>Shigella sonnei<\/jats:italic>\n            isolates, 16 (19.51%)\n            <jats:italic>Shigella flexneri<\/jats:italic>\n            isolates, 3 (3.65%)\n            <jats:italic>Shigella boydii<\/jats:italic>\n            isolates, and 2 (2.43%)\n            <jats:italic>Shigella dysenteriae<\/jats:italic>\n            isolates. In an antibiogram test, all\n            <jats:italic>Shigella<\/jats:italic>\n            strains were susceptible to ceftazidime, ciprofloxacin, and ceftriaxone. They showed high degrees of sensitivity to nalidixic acid, gentamicin, cephalothin, and amikacin. Approximately 90.24% of the\n            <jats:italic>Shigella<\/jats:italic>\n            isolates were resistant to co-trimoxazole. The plasmid profile patterns of all strains were determined by a modified alkaline lysis method. The average number of plasmid bands for each strain was 9.5. By plasmid profile analysis we identified 56 genotypes among all isolates and 42, 14, 3, and 2 genotypes among the\n            <jats:italic>S. sonnei<\/jats:italic>\n            ,\n            <jats:italic>S. flexneri<\/jats:italic>\n            ,\n            <jats:italic>S. boydii<\/jats:italic>\n            , and\n            <jats:italic>S. dysenteriae<\/jats:italic>\n            strains, respectively. PCR assays showed that all isolates were positive for two virulence genes,\n            <jats:italic>ipaBCD<\/jats:italic>\n            and\n            <jats:italic>ipaH<\/jats:italic>\n            . In conclusion, these data mandate local monitoring of drug resistance and its consideration in the empirical therapy of\n            <jats:italic>Shigella<\/jats:italic>\n            infections. These results also demonstrate that plasmid profile analysis is more reliable than antibiotic susceptibility pattern analysis for the identification of\n            <jats:italic>Shigella<\/jats:italic>\n            epidemic strains isolated in Iran.\n          <\/jats:p>","DOI":"10.1128\/jcm.00310-06","type":"journal-article","created":{"date-parts":[[2006,8,5]],"date-time":"2006-08-05T01:54:18Z","timestamp":1154742858000},"page":"2879-2883","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Characterization of\n            <i>Shigella<\/i>\n            Strains in Iran by Plasmid Profile Analysis and PCR Amplification of\n            <i>ipa<\/i>\n            Genes"],"prefix":"10.1128","volume":"44","author":[{"given":"Shohreh","family":"Farshad","sequence":"first","affiliation":[{"name":"Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz 71937, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raheleh","family":"Sheikhi","sequence":"additional","affiliation":[{"name":"Microbiology Department, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aziz","family":"Japoni","sequence":"additional","affiliation":[{"name":"Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz 71937, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezzatollah","family":"Basiri","sequence":"additional","affiliation":[{"name":"Microbiology Department, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolvahab","family":"Alborzi","sequence":"additional","affiliation":[{"name":"Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz 71937, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"87","volume":"57","year":"2003","unstructured":"Ahmetagic, S., E. 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