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The assay identified all haplotypes of<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>and five of six positive paraffin-embedded tissues. The assay sensitivity threshold was 1 pg genomic DNA of the mold form and 2 CFU of the yeast form of<jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>. No cross-reactivity was observed against other fungal DNA. 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