{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:04:37Z","timestamp":1787069077720,"version":"build-2736575974"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["NS34235"],"award-info":[{"award-number":["NS34235"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Limited data suggest that the cerebrospinal fluid\n                    <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n                    particle agglutination assay (CSF-TPPA) is sensitive and a CSF\n                    <jats:named-content content-type=\"genus-species\">Treponema pallidum<\/jats:named-content>\n                    hemagglutination assay (CSF-TPHA) titer of \u22651:640 is specific for neurosyphilis diagnosis. CSF-TPPA reactivity and titer were determined for a convenience sample of 191 CSF samples from individuals enrolled in a study of CSF abnormalities in syphilis (training data set). The sensitivity of a reactive test and the specificity for reactivity at serial higher CSF dilutions were determined. Subsequently, CSF-TPPA reactivity at a 1:640 dilution was determined for all available samples from study participants enrolled after the last training sample was collected (validation data set,\n                    <jats:italic>n<\/jats:italic>\n                    = 380). Neurosyphilis was defined as (i) a reactive CSF Venereal Disease Research Laboratory test (CSF-VDRL), (ii) detection of\n                    <jats:named-content content-type=\"genus-species\">T. pallidum<\/jats:named-content>\n                    in CSF by reverse transcriptase PCR, or (iii) new vision loss or hearing loss. In the training data set, the diagnostic sensitivities of a reactive CSF fluorescent treponemal antibody absorption test (CSF-FTA-ABS) and a reactive CSF-TPPA did not differ significantly (67 to 98% versus 76 to 95%). The specificity of a CSF-TPPA titer of \u22651:640 was significantly higher than that of lower dilutions and was not significantly different from that of CSF-VDRL. In the validation data set, the diagnostic specificity of a CSF-TPPA titer of \u22651:640 was high and did not differ significantly from that of CSF-VDRL (93 to 94% versus 90 to 91%). Ten CSF samples with a nonreactive CSF-VDRL had a CSF-TPPA titer of \u22651:640. If a CSF-TPPA titer of \u22651:640 was used in addition to a reactive CSF-VDRL, the number of neurosyphilis diagnoses would have increased from 47 to 57 (21.3%). A CSF-TPPA titer cutoff of \u22651:640 may be useful in identifying patients with neurosyphilis when CSF-VDRL is nonreactive.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00310-17","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T20:50:24Z","timestamp":1491425424000},"page":"1865-1870","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Cerebrospinal Fluid Treponema pallidum Particle Agglutination Assay for Neurosyphilis Diagnosis"],"prefix":"10.1128","volume":"55","author":[{"given":"Christina M.","family":"Marra","sequence":"first","affiliation":[{"name":"Department of Neurology, Harborview Medical Center, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare L.","family":"Maxwell","sequence":"additional","affiliation":[{"name":"Department of Neurology, Harborview Medical Center, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelia B.","family":"Dunaway","sequence":"additional","affiliation":[{"name":"Department of Medicine, Harborview Medical Center, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon K.","family":"Sahi","sequence":"additional","affiliation":[{"name":"Department of Neurology, Harborview Medical Center, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren C.","family":"Tantalo","sequence":"additional","affiliation":[{"name":"Department of Neurology, Harborview Medical Center, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr6404a1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181d877a1"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31824c0e62"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20147"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPWSL3G8RXMCQR"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1258\/0956462001915750"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000049"},{"key":"e_1_3_2_9_2","volume-title":"Neurosyphilis","author":"Merritt HH","year":"1946","unstructured":"Merritt HH Adams RD Solomon HC . 1946. Neurosyphilis. Oxford, New York, NY."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1978.03630260050016"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/381227"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e32830184df"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000230481.28936.e5"},{"key":"e_1_3_2_14_2","volume-title":"A manual of tests for syphilis","author":"Larsen SA","year":"1998","unstructured":"Larsen SA Pope V Johnson RE Kennedy EJ Jr . 1998. A manual of tests for syphilis, 9th ed. American Public Health Association, Washington, DC.","edition":"9"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00310-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00310-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T04:00:40Z","timestamp":1646452840000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00310-17"}},"subtitle":[],"editor":[{"given":"Erik","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,6]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1128\/JCM.00310-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00310-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6]]},"assertion":[{"value":"2017-02-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-03-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-05-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}