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Subsequent investigations identified a novel coronavirus, now named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), from the affected patients. Highly sensitive and specific laboratory diagnostics are important for controlling the rapidly evolving SARS-CoV-2-associated coronavirus disease 2019 (COVID-19) epidemic. In this study, we developed and compared the performance of three novel real-time reverse transcription-PCR (RT-PCR) assays targeting the RNA-dependent RNA polymerase (RdRp)\/helicase (Hel), spike (S), and nucleocapsid (N) genes of SARS-CoV-2 with that of the reported RdRp-P2 assay, which is used in &gt;30 European laboratories.<\/jats:p>","DOI":"10.1128\/jcm.00310-20","type":"journal-article","created":{"date-parts":[[2020,3,5]],"date-time":"2020-03-05T05:39:24Z","timestamp":1583386764000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":747,"title":["Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp\/Hel Real-Time Reverse Transcription-PCR Assay Validated\n                    <i>In Vitro<\/i>\n                    and with Clinical Specimens"],"prefix":"10.1128","volume":"58","author":[{"given":"Jasper 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