{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T10:07:09Z","timestamp":1779098829037,"version":"3.51.4"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n            is a tier 1 agent due to its contagious pneumopathogenicity, extremely rapid progression, and high mortality rate. As the disease is usually fatal without appropriate therapy, rapid detection from clinical matrices is critical to patient outcomes. We previously engineered the diagnostic phage \u03a6A1122 with\n            <jats:italic>luxAB<\/jats:italic>\n            to create a \u201clight-tagged\u201d reporter phage. \u03a6A1122::\n            <jats:italic>luxAB<\/jats:italic>\n            rapidly detects\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            in pure culture and human serum by transducing a bioluminescent signal response. In this report, we assessed the analytical specificity of the reporter phage and investigated diagnostic utility (detection and antibiotic susceptibility analysis) directly from spiked whole blood. The bioreporter displayed 100% (\n            <jats:italic>n<\/jats:italic>\n            = 59) inclusivity for\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            and consistent intraspecific signal transduction levels. False positives were not obtained from species typically associated with bacteremia or those relevant to plague diagnosis. However, some non-\n            <jats:named-content content-type=\"genus-species\">pestis Yersinia<\/jats:named-content>\n            strains and\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            did elicit signals, albeit at highly attenuated transduction levels. Diagnostic performance was assayed in simple broth-enriched blood samples and standard aerobic culture bottles. In blood, &lt;10\n            <jats:sup>2<\/jats:sup>\n            CFU\/ml was detected within 5 h. In addition,\n            <jats:named-content content-type=\"genus-species\">Y. pestis<\/jats:named-content>\n            was identified directly from positive blood cultures within 20 to 45 min without further processing. Importantly, coincubation of blood samples with antibiotics facilitated simultaneous antimicrobial susceptibility profiling. Consequently, the reporter phage demonstrated rapid detection and antibiotic susceptibility profiling directly from clinical samples, features that may improve patient prognosis during plague outbreaks.\n          <\/jats:p>","DOI":"10.1128\/jcm.00316-14","type":"journal-article","created":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T08:21:53Z","timestamp":1402561313000},"page":"2998-3003","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Rapid Detection and Simultaneous Antibiotic Susceptibility Analysis of Yersinia pestis Directly from Clinical Specimens by Use of Reporter Phage"],"prefix":"10.1128","volume":"52","author":[{"given":"J. P.","family":"Vandamm","sequence":"first","affiliation":[{"name":"Guild BioSciences, Charleston, South Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Rajanna","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. J.","family":"Sharp","sequence":"additional","affiliation":[{"name":"Guild BioSciences, Charleston, South Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. J.","family":"Molineux","sequence":"additional","affiliation":[{"name":"Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. A.","family":"Schofield","sequence":"additional","affiliation":[{"name":"Guild BioSciences, Charleston, South Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00306-06"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199709043371004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0701.010106"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268811001245"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0201.960103"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/604718"},{"key":"e_1_3_3_8_2","volume-title":"Performance standards for antimicrobial susceptibility testing; 20th informational supplement","author":"Clinical and Laboratory Standards Institute","year":"2010","unstructured":"Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing; 20th informational supplement. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_9_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard\u20148th ed.","author":"Clinical and Laboratory Standards Institute","year":"2009","unstructured":"Clinical and Laboratory Standards Institute. 2009. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard\u20148th ed. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00339-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.32607\/20758251-2012-4-3-46-58"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025486"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.17.5248-5262.2003"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01533-09"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2012.04.019"},{"key":"e_1_3_3_16_2","first-page":"493","article-title":"Yersinia pestis infection in Vietnam. II","volume":"133","author":"Butler T","year":"1976","unstructured":"ButlerTLevinJLinhNNChauDMAdickmanMArnoldK. 1976. Yersinia pestis infection in Vietnam. II. Quantitative blood cultures and detection of endotoxin in the cerebrospinal fluid of patients with meningitis. J. Infect. 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