{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:49:02Z","timestamp":1790344142476,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100021806","name":"Hsinchu Science Park Bureau, Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NSTC 112-2314-B-037-054-MY2"],"award-info":[{"award-number":["NSTC 112-2314-B-037-054-MY2"]}],"id":[{"id":"10.13039\/501100021806","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011645","name":"Kaohsiung Medical University Chung-Ho Memorial Hospital","doi-asserted-by":"publisher","award":["KMUH113-3R25"],"award-info":[{"award-number":["KMUH113-3R25"]}],"id":[{"id":"10.13039\/501100011645","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004694","name":"Kaohsiung Medical University","doi-asserted-by":"publisher","award":["KMU-TB114001"],"award-info":[{"award-number":["KMU-TB114001"]}],"id":[{"id":"10.13039\/501100004694","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,6,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      This study aimed to evaluate the\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activity of isavuconazole and ravuconazole against clinical\n                      <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                      isolates. Additionally, we assessed the performance of the Sensititre YeastOne (SYO) assay compared to the reference Clinical and Laboratory Standards Institute (CLSI) broth microdilution (BMD) method for antifungal susceptibility testing. A total of 267\n                      <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                      clinical isolates were collected from multiple centers in Taiwan between 2008 and 2020. The MIC values for amphotericin B, fluconazole, itraconazole, posaconazole, and voriconazole obtained using the CLSI BMD were compared to those determined using SYO. Among the seven antifungal agents tested using the CLSI BMD method,\n                      <jats:italic toggle=\"yes\">T. asahii<\/jats:italic>\n                      isolates (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 224) exhibited significantly higher MICs for fluconazole, voriconazole, isavuconazole, and ravuconazole compared to non-\n                      <jats:italic toggle=\"yes\">T<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">asahii<\/jats:italic>\n                      isolates (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 43) (\n                      <jats:italic toggle=\"yes\">p<\/jats:italic>\n                      &lt; 0.05). Across all isolates, voriconazole demonstrated the lowest geometric mean (GM) MIC (0.06 mg\/L). For\n                      <jats:italic toggle=\"yes\">T. asahii<\/jats:italic>\n                      , the MIC\u2085\u2080, MIC\u2089\u2080, and GM MICs for posaconazole, isavuconazole, and ravuconazole were within \u00b11 twofold dilution, while mode MICs showed slightly greater variation (MIC\u2085\u2080, MIC\u2089\u2080, mode, and GM MICs were 0.25, 0.5, 0.5, and 0.28 mg\/L; 0.12, 0.5, 0.12, and 0.17 mg\/L; and 0.25, 0.5, 0.25, and 0.21 mg\/L, respectively). The consistency of the MIC measurements between the SYO and CLSI BMD methods was assessed for amphotericin B, fluconazole, itraconazole, posaconazole, and voriconazole. The highest agreement within \u00b11 twofold dilution for\n                      <jats:italic toggle=\"yes\">T. asahii<\/jats:italic>\n                      isolates was observed for posaconazole (94.2%). The essential agreement within \u00b12 twofold dilutions between both methods for all drugs was &gt;97%. In conclusion, isavuconazole and ravuconazole demonstrated favorable\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      activity against clinical\n                      <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                      isolates, while the SYO assay showed good concordance with the CLSI BMD method.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Invasive infections caused by\n                        <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                        species pose significant therapeutic challenges, primarily due to their intrinsic resistance to echinocandins and the limited availability of effective treatment options. This study provides essential data on the\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        activity of newer azoles and comprehensively evaluates the performance and concordance of the Sensititre YeastOne (SYO) and CLSI broth microdilution (BMD) methods. The current study analyzed 267\n                        <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                        clinical isolates collected from multicenter settings in Taiwan. Our results demonstrate that both isavuconazole and ravuconazole exhibit favorable\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        activities against\n                        <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                        species. For\n                        <jats:italic toggle=\"yes\">T. asahii<\/jats:italic>\n                        , the essential agreement between the SYO and CLSI BMD methods exceeded 97% for all tested antifungal agents, indicating that the SYO method exhibits good concordance for most\n                        <jats:italic toggle=\"yes\">Trichosporon<\/jats:italic>\n                        species. Further investigations are warranted to validate these findings and to assess their clinical implications.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00319-25","type":"journal-article","created":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T09:00:46Z","timestamp":1747040446000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["<i>In vitro<\/i>\n                    activity of isavuconazole, ravuconazole, and comparison of the Sensititre YeastOne and CLSI broth microdilution methods against clinical isolates of\n                    <i>Trichosporon<\/i>\n                    species"],"prefix":"10.1128","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2175-582X","authenticated-orcid":false,"given":"Shih-Hao","family":"Lo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Internal Medicine, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University","place":["Kaohsiung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ting","family":"Tseng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University","place":["Kaohsiung, 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Hospital, College of Medicine, Chang Gung University","place":["Kaohsiung, Taiwan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Po-Liang","family":"Lu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University","place":["Kaohsiung, Taiwan"]},{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of post-baccalaureate Medicine, Kaohsiung Medical University","place":["Kaohsiung, Taiwan"]},{"id":[{"id":"https:\/\/ror.org\/03gk81f96","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Liquid Biopsy and Cohort Research, Kaohsiung Medical University","place":["Kaohsiung, 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