{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:23:55Z","timestamp":1792607035717,"version":"4.5.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Multilocus sequence typing (MLST) was used to characterize the genetic profiles of 51\n            <jats:italic>Candida albicans<\/jats:italic>\n            isolates collected from 12 hospitals in Taiwan. Among the 51 isolates, 16 were epidemiologically unrelated, 28 were isolates from 11 critically ill, human immunodeficiency virus (HIV)-negative patients, and 7 were long-term serial isolates from 3 HIV-positive patients. Internal regions of seven housekeeping genes were sequenced. A total of 83 polymorphic nucleotide sites were identified. Ten to 20 different genotypes were observed at the different loci, resulting, when combined, in 45 unique genotype combinations or diploid sequence types (DSTs). Thirty (36.1%) of the 83 individual changes were synonymous and 53 (63.9%) were nonsynonymous. Due to the diploid nature of\n            <jats:italic>C. albicans<\/jats:italic>\n            , MLST was more discriminatory than the pulsed-field gel electrophoresis-BssHII-restricted fragment method in discriminating epidemiologically related strains. MLST is able to trace the microevolution over time of\n            <jats:italic>C. albicans<\/jats:italic>\n            isolates in the same patient. All but one of the DSTs of our Taiwanese strain collections were novel to the internet\n            <jats:italic>C. albicans<\/jats:italic>\n            DST database (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"url\" xlink:href=\"http:\/\/test1.mlst.net\/\">http:\/\/test1.mlst.net\/<\/jats:ext-link>\n            ). The DSTs of\n            <jats:italic>C. albicans<\/jats:italic>\n            in Taiwan were analyzed together with those of the reference strains and of the strains from the United Kingdom and United States by unweighted-pair group method using average linkages and minimum spanning tree. Our result showed that the DNA type of each isolate was patient specific and associated with ABC type and decade of isolation but not associated with mating type, anatomical source of isolation, hospital origin, or fluconazole resistance patterns.\n          <\/jats:p>","DOI":"10.1128\/jcm.00320-06","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T10:58:07Z","timestamp":1149677887000},"page":"2172-2178","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Multilocus Sequence Typing for Analyses of Clonality of\n            <i>Candida albicans<\/i>\n            Strains in Taiwan"],"prefix":"10.1128","volume":"44","author":[{"given":"Kuo-Wei","family":"Chen","sequence":"first","affiliation":[{"name":"Laboratory for Mycology, Division of Research and Diagnostics, Center for Disease Control, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee-Chun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsiu-Jung","family":"Lo","sequence":"additional","affiliation":[{"name":"Division of Clinical Research, National Health Research Institutes, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank C.","family":"Odds","sequence":"additional","affiliation":[{"name":"Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory for Mycology, Division of Research and Diagnostics, Center for Disease Control, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Yang","family":"Lin","sequence":"additional","affiliation":[{"name":"Laboratory for Mycology, Division of Research and Diagnostics, Center for Disease Control, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory for Mycology, Division of Research and Diagnostics, Center for Disease Control, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1673-1679.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1290-1297.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5265-5266.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45829-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg275"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.2307\/30141234"},{"key":"e_1_3_2_8_2","first-page":"791","volume":"100","year":"2001","unstructured":"Chen, Y. 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