{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T23:50:01Z","timestamp":1769817001434,"version":"3.49.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Loop-mediated isothermal amplification (LAMP) is a novel method that rapidly amplifies target DNA with high specificity under isothermal conditions. It has been applied as a diagnostic tool for several infectious diseases, including viral, bacterial, and parasitic diseases. In the present study, we developed a LAMP method for the molecular diagnosis of<jats:italic>Plasmodium knowlesi<\/jats:italic>infection (<jats:italic>Pk<\/jats:italic>LAMP) and evaluated its sensitivity, specificity, and clinical applicability. We designed three sets of<jats:italic>Pk<\/jats:italic>LAMP primers for the species-specific \u03b2-tubulin gene. The primer sets for<jats:italic>Pk<\/jats:italic>LAMP specifically amplified the autologous DNA extracts of<jats:italic>P. knowlesi<\/jats:italic>, and the sensitivity of the test was 100-fold that of single-PCR assay. These results indicate that our<jats:italic>Pk<\/jats:italic>LAMP method can be used to efficiently distinguish between<jats:italic>P. knowlesi<\/jats:italic>and other malaria parasites. To evaluate the feasibility of using<jats:italic>in vivo<\/jats:italic>materials, comparisons of<jats:italic>Pk<\/jats:italic>LAMP and the conventional nested PCR (nPCR) method and microscopic examination were made with blood samples from two experimentally infected monkeys. These studies showed that<jats:italic>P. knowlesi<\/jats:italic>infection can be identified much earlier with<jats:italic>Pk<\/jats:italic>LAMP than with nPCR and microscopy. Moreover, the detection performance of<jats:italic>Pk<\/jats:italic>LAMP using whole blood as the template was identical to that of<jats:italic>Pk<\/jats:italic>LAMP when genomic DNA extracts were used. These results suggest that the<jats:italic>Pk<\/jats:italic>LAMP method is a promising tool for molecular diagnosis of<jats:italic>P. knowlesi<\/jats:italic>infection in areas of endemicity.<\/jats:p>","DOI":"10.1128\/jcm.00331-10","type":"journal-article","created":{"date-parts":[[2010,5,6]],"date-time":"2010-05-06T01:53:47Z","timestamp":1273110827000},"page":"2509-2514","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Evaluation of a Loop-Mediated Isothermal Amplification Method as a Tool for Diagnosis of Infection by the Zoonotic Simian Malaria Parasite<i>Plasmodium knowlesi<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Hiroshi","family":"Iseki","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Kawai","sequence":"additional","affiliation":[{"name":"Center for Tropical Medicine and Parasitology, Dokkyo Medical University, Mibu, Tochigi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Takahashi","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Hirai","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Tanabe","sequence":"additional","affiliation":[{"name":"Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoaki","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Igarashi","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1520\/JFS13607J","volume":"39","year":"1994","unstructured":"Akane, A., H. 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