{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:43:50Z","timestamp":1791251030617,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Leptospirosis is a worldwide-distributed zoonosis, endemic in tropical areas. Epidemiologic investigations of leptospirosis still rely on tedious serological identification tests. Recently, molecular typing systems based on variable-number tandem-repeat (VNTR) analysis have been described and have been used to identify\n            <jats:italic>Leptospira interrogans<\/jats:italic>\n            strains. Although\n            <jats:italic>L. interrogans<\/jats:italic>\n            is the most common\n            <jats:italic>Leptospira<\/jats:italic>\n            species encountered in human infections around the world, other pathogenic species, such as\n            <jats:italic>Leptospira kirschneri<\/jats:italic>\n            and\n            <jats:italic>Leptospira borgpetersenii<\/jats:italic>\n            , are also frequently associated with human leptospirosis. In this study, we aimed to extend multilocus VNTR analysis (MLVA) identification of strains to species other than\n            <jats:italic>L. interrogans<\/jats:italic>\n            . We designed primers for VNTR loci found in\n            <jats:italic>L. interrogans<\/jats:italic>\n            ,\n            <jats:italic>L. kirschneri<\/jats:italic>\n            , and\n            <jats:italic>L. borgpetersenii<\/jats:italic>\n            . The discriminatory power of the redefined primers was evaluated on collection strains and then on clinical strains. We also carried out a retrospective study on 156 strains isolated from patients and animals from New Caledonia, an area of high endemicity in the South Pacific. Our results show that this simple PCR-based MLVA typing technique is a powerful methodology for the epidemiology of leptospirosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.00336-06","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T18:13:36Z","timestamp":1162836816000},"page":"3954-3962","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Application of Multilocus Variable-Number Tandem-Repeat Analysis for Molecular Typing of the Agent of Leptospirosis"],"prefix":"10.1128","volume":"44","author":[{"given":"Laurence","family":"Salau\u0308n","sequence":"first","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"},{"name":"Laboratoire de Recherche en Bacte\u0301riologie, Institut Pasteur de Nouvelle-Cale\u0301donie, BP61, 98845 Noume\u0301a, Nouvelle-Cale\u0301donie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Me\u0301rien","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche en Bacte\u0301riologie, Institut Pasteur de Nouvelle-Cale\u0301donie, BP61, 98845 Noume\u0301a, Nouvelle-Cale\u0301donie"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Gurianova","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology, Saint Petersburg Pasteur Institute, Saint Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Baranton","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.573"},{"key":"e_1_3_2_3_2","first-page":"51","volume":"92","year":"1999","unstructured":"Bouree, P., L. 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