{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:07:35Z","timestamp":1792674455623,"version":"4.5.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Allergiestiftung Ulrich M\u00fcller-Gierok"},{"name":"AGS-PDF postdoctoral fellowship, Agency of Science and Technology, Singapore"},{"name":"Deutsche Stiftung Dermatologie"},{"DOI":"10.13039\/501100006693","name":"Arbeitsgemeinschaft Dermatologische Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006693","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008578","name":"Jubil\u00e4umsstiftung der Schweizerischen Lebensversicherungs- und Rentenanstalt f\u00fcr Volksgesundheit und medizinische Forschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008578","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            is a genus of lipid-dependent yeasts. It is associated with common skin diseases such as pityriasis versicolor and atopic dermatitis and can cause systemic infections in immunocompromised individuals. Owing to the slow growth and lipid requirements of these fastidious yeasts, convenient and reliable antifungal drug susceptibility testing assays for\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            spp. are not widely available. Therefore, we optimized a broth microdilution assay for the testing of\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            that is based on the CLSI and EUCAST assays for\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            and other yeasts. The addition of ingredients such as lipids and esculin provided a broth medium formulation that enabled the growth of all\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            spp. and could be read, with the colorimetric indicator resazurin, by visual and fluorescence readings. We tested the susceptibility of 52 strains of 13\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            species to 11 commonly used antifungals. MIC values determined by visual readings were in good agreement with MIC values determined by fluorescence readings. The lowest MICs were found for the azoles itraconazole, posaconazole, and voriconazole, with MIC\n            <jats:sub>90<\/jats:sub>\n            values of 0.03 to 1.0 \u03bcg\/ml, 0.06 to 0.5 \u03bcg\/ml, and 0.03 to 2.0 \u03bcg\/ml, respectively. All\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            spp. were resistant to echinocandins and griseofulvin. Some\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            spp. also showed high MIC values for ketoconazole, which is the most widely recommended topical antifungal to treat\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            skin infections. In summary, our assay enables the fast and reliable susceptibility testing of\n            <jats:named-content content-type=\"genus-species\">Malassezia<\/jats:named-content>\n            spp. with a large panel of different antifungals.\n          <\/jats:p>","DOI":"10.1128\/jcm.00338-17","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T00:50:24Z","timestamp":1491439824000},"page":"1883-1893","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Antifungal Susceptibility Testing of Malassezia spp. with an Optimized Colorimetric Broth Microdilution Method"],"prefix":"10.1128","volume":"55","author":[{"given":"Cheryl","family":"Leong","sequence":"first","affiliation":[{"name":"Department of Dermatology, University and University Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonino","family":"Buttafuoco","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University and University Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Glatz","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University and University Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp P.","family":"Bosshard","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University and University Hospital of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003892"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12171"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mmcr.2014.04.004"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2009.01814.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2012.01.007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12614"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myu010"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3589-3593.2004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.09.016"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2000.03294.x"},{"key":"e_1_3_3_12_2","volume-title":"Reference methods for broth dilution antifungal susceptibility testing of yeasts; approved standard\u20143rd ed","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. 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