{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:48:44Z","timestamp":1790329724795,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Staphylococcus lugdunensis<\/jats:italic>\n            is an aggressive, virulent member of the coagulase-negative staphylococci (CoNS) that is responsible for severe, rapidly progressive skin and soft tissue infections and native valve endocarditis. To facilitate prompt identification and appropriate therapy, we describe here a rapid and robust multiplex real-time PCR assay that is able to definitively distinguish\n            <jats:italic>S. lugdunensis<\/jats:italic>\n            from other staphylococci. Using melting curve analysis, the assay also identifies\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            and CoNS other than\n            <jats:italic>S. lugdunensis<\/jats:italic>\n            and determines MecA-dependent resistance to methicillin (meticillin). When applied to a panel of well-characterized staphylococcal reference strains, as well as 165 clinical isolates previously identified by conventional methods, the assay was both sensitive and specific for\n            <jats:italic>S. lugdunensis<\/jats:italic>\n            , correctly identifying the reference strain and all 47\n            <jats:italic>S. lugdunensis<\/jats:italic>\n            isolates without inappropriate amplification of other staphylococci. Furthermore, rapid biochemical identification using the WEE-TAB system to detect ornithine decarboxylase activity was found to be unsuitable as an alternative to PCR identification, displaying just 31% sensitivity and 77% specificity when tested on a subset (90 isolates) of the clinical strains. We therefore propose that this simple, accurate PCR approach will allow for the routine and timely identification of\n            <jats:italic>S. lugdunensis<\/jats:italic>\n            in the clinical microbiology laboratory.\n          <\/jats:p>","DOI":"10.1128\/jcm.00342-09","type":"journal-article","created":{"date-parts":[[2009,9,10]],"date-time":"2009-09-10T01:32:15Z","timestamp":1252546335000},"page":"3472-3477","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Comparison of Real-Time PCR and Conventional Biochemical Methods for Identification of\n            <i>Staphylococcus lugdunensis<\/i>"],"prefix":"10.1128","volume":"47","author":[{"given":"Benjamin A.","family":"Pinsky","sequence":"first","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divinia","family":"Samson","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, California 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laleh","family":"Ghafghaichi","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, California 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen J.","family":"Baron","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California 94305"},{"name":"Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, California 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niaz","family":"Banaei","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California 94305"},{"name":"Clinical Microbiology Laboratory, Stanford Hospital and Clinics, Palo Alto, California 94304"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.07.011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01024-08"},{"key":"e_1_3_2_4_2","unstructured":"Performance standards for antimicrobial susceptibility testing 19th informational supplement. 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