{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:07:19Z","timestamp":1791158839289,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n We report the characteristics of 115 extended-spectrum beta-lactamase (ESBL)-producing\n Escherichia coli<\/jats:named-content>\n clinical isolates, from 115 unique Danish patients, over a 1-year study interval (1 October 2008 to 30 September 2009). Forty-four (38%) of the ESBL isolates represented sequence type 131 (ST13)1, from phylogenetic group B2. The remaining 71 isolates were from phylogenetic groups D (27%), A (22%), B1 (10%), and B2 (3%). Serogroup O25 ST131 isolates (\n n<\/jats:italic>\n = 42; 95% of ST131) comprised 7 different K antigens, whereas two ST131 isolates were O16:K100:H5. Compared to non-ST131 isolates, ST131 isolates were associated positively with CTX-M-15 and negatively with CTX-M-1 and CTX-M-14. They also were associated positively with 11 virulence genes, including\n afa<\/jats:italic>\n and\n dra<\/jats:italic>\n (Dr family adhesins), the F10\n papA<\/jats:italic>\n allele (P fimbria variant),\n fimH<\/jats:italic>\n (type 1 fimbriae),\n fyuA<\/jats:italic>\n (yersiniabactin receptor),\n iha<\/jats:italic>\n (adhesin siderophore),\n iutA<\/jats:italic>\n (aerobactin receptor),\n kpsM<\/jats:italic>\n II (group 2 capsules),\n malX<\/jats:italic>\n (pathogenicity island marker),\n ompT<\/jats:italic>\n (outer membrane protease),\n sat<\/jats:italic>\n (secreted autotransporter toxin), and\n usp<\/jats:italic>\n (uropathogenicity-specific protein) and negatively with\n hra<\/jats:italic>\n (heat-resistant agglutinin) and\n iroN<\/jats:italic>\n (salmochelin receptor). The consensus virulence gene profile (>90% prevalence) of the ST131 isolates included\n fimH<\/jats:italic>\n ,\n fyuA<\/jats:italic>\n ,\n malX<\/jats:italic>\n , and\n usp<\/jats:italic>\n (100% each),\n ompT<\/jats:italic>\n and the F10\n papA<\/jats:italic>\n allele (95% each), and\n kpsM<\/jats:italic>\n II and\n iutA<\/jats:italic>\n (93% each). ST131 isolates were also positively associated with community acquisition, extraintestinal pathogenic\n E. coli<\/jats:named-content>\n (ExPEC) status, and the O25, K100, and H4 antigens. Thus, among ESBL\n E. coli<\/jats:named-content>\n isolates in Copenhagen, ST131 was the most prevalent clonal group, was community associated, and exhibited distinctive and comparatively extensive virulence profiles, plus a greater variety of capsular antigens than reported previously.\n <\/jats:p>","DOI":"10.1128\/jcm.00346-13","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T06:34:49Z","timestamp":1365057289000},"page":"1779-1785","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Prevalence and Characteristics of the Epidemic Multiresistant Escherichia coli ST131 Clonal Group among Extended-Spectrum Beta-Lactamase-Producing E. coli Isolates in Copenhagen, Denmark"],"prefix":"10.1128","volume":"51","author":[{"given":"Bente","family":"Olesen","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Hiller\u00f8d Hospital, Hiller\u00f8d, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis S.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hiller\u00f8d Hospital, Hiller\u00f8d, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frida","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hiller\u00f8d Hospital, Hiller\u00f8d, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Frimodt-M\u00f8ller","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Hiller\u00f8d Hospital, Hiller\u00f8d, Denmark"},{"name":"Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke Fleron","family":"Leihof","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Struve","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Reference and Research on Escherichia and Klebsiella, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flemming","family":"Scheutz","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Reference and Research on Escherichia and Klebsiella, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Johnston","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen A.","family":"Krogfelt","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for Reference and Research on Escherichia and Klebsiella, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq415"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.11.003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00025-11"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-137-10-200211190-00007"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0580-9517(08)70447-1"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.18.3.775-779.1977"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.11.2559-2564.1989"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2011.03.018"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02421-09"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.06.007"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00297-09"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2618-2622.2004"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.02.007"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/315217"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1587-1599.2000"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2161-2168.2003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.4.1773-1776.2004"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir757"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.08.007"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.59"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01338-09"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq491"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05824-11"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1764-x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2009.02465.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06921-11"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks278"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr349"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1101.040418"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4667-4670.2005"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.5860-5864.2005"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00230-09"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks477"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01909-12"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01376-12"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00346-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00346-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T20:43:36Z","timestamp":1646426616000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00346-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1128\/JCM.00346-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00346-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6]]},"assertion":[{"value":"2013-02-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}