{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:00:14Z","timestamp":1778778014049,"version":"3.51.4"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2023,10,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>This study aimed to evaluate different serological strategies for the postnatal diagnosis of congenital toxoplasmosis (CT) and establish a biological algorithm for CT diagnosis. The study analyzed serological data of immunoglobulins M, A, and G (IgM, IgA, IgG) performed by immunoenzymatic and compared immunological profile (CIP) assays in 668 newborns with CT diagnosis across four testing periods: P1 (D0\u2013 D10), P2 (D11\u2013D35), P3 (D36\u2013D45), and P4 (&gt;D45). Forty-nine percent of the 668 CT cases were diagnosed during P1 and 34%, 4%, and 12% during P2, P3, and P4, respectively. CIP assays detected neosynthetized IgMs\/IgGs in 98% of CT cases diagnosed during P1, while IgMs and IgAs were detected in 90% and 57% of CT cases diagnosed during P2 and in 88% and 67% of diagnoses made during P3, respectively. Detection of neosynthesized IgMs\/IgGs, IgMs, and IgAs by immunoassay contributed to CT diagnosis in 81%, 77%, and 60% of cases, respectively. In total, 46% of serum samples were positive for all three parameters, 27% for two, and 27% for one of the three. The study recommends using the CIP assay as standard during P1 for CT diagnosis and IgM and IgA immunoassays after P1. A clinical and biological follow-up in a specialized center with a close collaboration between biologists and clinicians is highly recommended to increase the chances of early diagnosis. Overall, this study provides useful information for the development of a biological algorithm for CT diagnosis, which can aid in early detection and appropriate treatment of this disease.<\/jats:p>","DOI":"10.1128\/jcm.00354-23","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T15:25:26Z","timestamp":1695223526000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Contribution of serology in congenital toxoplasmosis diagnosis: results from a 10-year French retrospective study"],"prefix":"10.1128","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6294-9338","authenticated-orcid":false,"given":"Julie","family":"Denis","sequence":"first","affiliation":[{"name":"Institut de Parasitologie et de Pathologie Tropicale, UR7292 Dynamique des interactions h\u00f4te pathog\u00e8ne, F\u00e9d\u00e9ration de M\u00e9decine Transrationnelle, Universit\u00e9 de Strasbourg , Strasbourg, France"},{"name":"Laboratoire de Parasitologie et Mycologie M\u00e9dicale, H\u00f4pitaux Universitaires de Strasbourg , Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Philippe","family":"Lemoine","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, CHU d'Angers , Angers, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Coralie","family":"L'ollivier","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence Toxoplasmose-P\u00f4le s\u00e9rologie, H\u00f4pitaux Universitaires de Strasbourg , Strasbourg, France"},{"name":"IHU-M\u00e9diterran\u00e9e Infection, Assistance Publique H\u00f4pitaux de Marseille (AP-HM) , Marseille, France"},{"name":"Aix Marseille University, IRD, AP-HM, SSA, VITROME, IHU M\u00e9diterran\u00e9e , Marseille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Sophie","family":"Deleplancque","sequence":"additional","affiliation":[{"name":"Centre National de R\u00e9f\u00e9rence Toxoplasmose-P\u00f4le s\u00e9rologie, H\u00f4pitaux Universitaires de Strasbourg , Strasbourg, France"},{"name":"CHU Lille, Parasitology Mycology Department, Lille University, Inserm, U995 - 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