{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:36:58Z","timestamp":1788572218496,"version":"build-2803163510"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Scrub typhus, caused by antigenically disparate isolates of\n                    <jats:named-content content-type=\"genus-species\">Orientia tsutsugamushi<\/jats:named-content>\n                    , is a widely distributed mite-borne human disease in the Asia Pacific region. Information regarding the heterogeneity of the immunodominant 56-kDa type-specific antigen (TSA) gene is crucial for the design and evaluation of scrub typhus-specific diagnostic assays and vaccines. Using indirect immunofluorescence assays (IFA) and PCR assays,\n                    <jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>\n                    was detected samples from rodents and patients with fever of unknown origin obtained from six provinces of Thailand during 2004 to 2007. Sequences were determined for a fragment of the 56-kDa TSA gene, and the relationship between these sequences and those previously determined were assessed. The phylogenetic analyses of partial 56-kDa TSA gene sequences demonstrated wide diversity and distribution of\n                    <jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>\n                    genotypes in Thailand. Furthermore, the genetic diversity grouped the scrub typhus agents into two commonly and five infrequently found genotypes within six provinces of Thailand. The two most commonly found genotypes of\n                    <jats:named-content content-type=\"genus-species\">O. tsutsugamushi<\/jats:named-content>\n                    described in this study do not associate with the prototype strains that are widely used for the design and evaluation of diagnostic assays and vaccine candidates. Thus, these new genotypes should be considered for future scrub typhus assay and vaccine development.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00355-11","type":"journal-article","created":{"date-parts":[[2011,5,18]],"date-time":"2011-05-18T23:55:42Z","timestamp":1305762942000},"page":"2584-2589","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Genotype Diversity and Distribution of Orientia tsutsugamushi Causing Scrub Typhus in Thailand"],"prefix":"10.1128","volume":"49","author":[{"given":"Toon","family":"Ruang-areerate","sequence":"first","affiliation":[{"name":"Research Division, Armed Forces Research Institute of Medical Science, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pimmada","family":"Jeamwattanalert","sequence":"additional","affiliation":[{"name":"Department of Enteric Disease, Armed Forces Research Institute of Medical Science, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuttikorn","family":"Rodkvamtook","sequence":"additional","affiliation":[{"name":"Research Division, Armed Forces Research Institute of Medical Science, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allen L.","family":"Richards","sequence":"additional","affiliation":[{"name":"Viral and Rickettsial Diseases Department, U.S. Naval Medical Research Center, Silver Spring, Maryland 20910"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piyanate","family":"Sunyakumthorn","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State, University, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jariyanart","family":"Gaywee","sequence":"additional","affiliation":[{"name":"Research Division, Armed Forces Research Institute of Medical Science, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/510585"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2007.00375.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-112-28107"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.3.3.4009"},{"key":"e_1_3_2_7_2","author":"Clark D. 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