{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:25:06Z","timestamp":1791307506687,"version":"4.3.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The close phylogenetic relationship of the important pathogen\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            and several species of commensal streptococci, particularly\n            <jats:named-content content-type=\"genus-species\">Streptococcus mitis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Streptococcus pseudopneumoniae<\/jats:named-content>\n            , and the recently demonstrated sharing of genes and phenotypic traits previously considered specific for\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            hamper the exact identification of\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            . Based on sequence analysis of 16S rRNA genes of a collection of 634 streptococcal strains, identified by multilocus sequence analysis, we detected a cytosine at position 203 present in all 440 strains of\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            but replaced by an adenosine residue in all strains representing other species of mitis group streptococci. The\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            -specific sequence signature could be demonstrated by sequence analysis or indirectly by restriction endonuclease digestion of a PCR amplicon covering the site. The\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            -specific signature offers an inexpensive means for validation of the identity of clinical isolates and should be used as an integrated marker in the annotation procedure employed in 16S rRNA-based molecular studies of complex human microbiotas. This may avoid frequent misidentifications such as those we demonstrate to have occurred in previous reports and in reference sequence databases.\n          <\/jats:p>","DOI":"10.1128\/jcm.00365-12","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T03:29:56Z","timestamp":1332473396000},"page":"1968-1973","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Novel Molecular Method for Identification of Streptococcus pneumoniae Applicable to Clinical Microbiology and 16S rRNA Sequence-Based Microbiome Studies"],"prefix":"10.1128","volume":"50","author":[{"given":"Christian F. 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