{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:03:54Z","timestamp":1784192634930,"version":"3.55.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Pathogen detection is critical to the process of generating and testing powdered infant formula (PIF). An obstacle associated with PIF microbial surveillance is that most current procedures are time-consuming and labor-intensive. We have developed a rapid, DNA microarray-based detection technique to identify 10 different pathogenic bacteria associated with PIF contamination based on the 16S-23S rRNA gene internal transcribed spacer (ITS) sequences and\n            <jats:italic>wzy<\/jats:italic>\n            (O antigen polymerase) gene. Using this procedure,\n            <jats:italic>Enterobacter sakazakii<\/jats:italic>\n            ,\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            ,\n            <jats:italic>Klebsiella oxytoca<\/jats:italic>\n            ,\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            ,\n            <jats:italic>Acinetobacter baumannii<\/jats:italic>\n            ,\n            <jats:italic>Bacillus cereus<\/jats:italic>\n            ,\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            ,\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            , and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157 were identified. One hundred eighty-five strains were used to validate the microarray assay (including 134 target pathogen strains and 51 closely related bacteria). Twenty-seven probes reproducibly detected multiple pathogens with high specificity and sensitivity (0.100 ng genomic DNA or 10\n            <jats:sup>4<\/jats:sup>\n            CFU\/ml). Twenty-one real PIF samples were tested by the microarray with 100% accuracy. The data presented reveal that the designed oligonucleotide microarray is a promising method for basic microbiology, clinical diagnosis, food safety, and epidemiological surveillance.\n          <\/jats:p>","DOI":"10.1128\/jcm.00366-09","type":"journal-article","created":{"date-parts":[[2009,7,30]],"date-time":"2009-07-30T01:28:53Z","timestamp":1248917333000},"page":"3178-3184","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Detection of\n            <i>Enterobacter sakazakii<\/i>\n            and Other Pathogens Associated with Infant Formula Powder by Use of a DNA Microarray"],"prefix":"10.1128","volume":"47","author":[{"given":"Min","family":"Wang","sequence":"first","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Nankai University, Tianjin 300071, China"},{"name":"Tianjin State Laboratory of Microbial Functional Genomics, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 HongDa Street, TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyang","family":"Cao","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Nankai University, Tianjin 300071, China"},{"name":"Tianjin State Laboratory of Microbial Functional Genomics, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 HongDa Street, TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qili","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Entry-Exit Inspection and Quarantine Bureau, 8 ZhaoFa Residential Quarter, 2nd St., TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamin","family":"Sun","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Nankai University, Tianjin 300071, China"},{"name":"Tianjin State Laboratory of Microbial Functional Genomics, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 HongDa Street, TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin Entry-Exit Inspection and Quarantine Bureau, 8 ZhaoFa Residential Quarter, 2nd St., TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Feng","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Nankai University, Tianjin 300071, China"},{"name":"Tianjin State Laboratory of Microbial Functional Genomics, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Biochip Corporation, 23 HongDa Street, TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Nankai University, Tianjin 300071, China"},{"name":"Tianjin State Laboratory of Microbial Functional Genomics, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Research Center for Functional Genomics and Biochips, 23 HongDa Street, TEDA, Tianjin 300457, China"},{"name":"Tianjin Biochip Corporation, 23 HongDa Street, TEDA, Tianjin 300457, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1.1.51"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(03)00035-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/4462"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/524737"},{"key":"e_1_3_2_6_2","first-page":"171","volume":"179","year":"2002","unstructured":"Chun, J., I. 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