{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:04:18Z","timestamp":1790316258008,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Rickettsia rickettsii<\/jats:italic>, the causative agent of Rocky Mountain spotted fever, is found throughout the Americas, where it is associated with different animal reservoirs and tick vectors. No molecular typing system currently exists to allow for the robust differentiation of isolates of<jats:italic>R. rickettsii<\/jats:italic>. Analysis of eight completed genome sequences of rickettsial species revealed a high degree of sequence conservation within the coding regions of chromosomes in the genus. Intergenic regions between coding sequences should be under less selective pressure to maintain this conservation and thus should exhibit greater nucleotide polymorphisms. Utilizing these polymorphisms, we developed a molecular typing system that allows for the genetic differentiation of isolates of<jats:italic>R. rickettsii<\/jats:italic>. This typing system was applied to a collection of 38 different isolates collected from humans, animals, and tick vectors from different geographic locations. Serotypes 364D, from<jats:italic>Dermacentor occidentalis<\/jats:italic>ticks, and Hlp, from<jats:italic>Haemaphysalis leporispalustris<\/jats:italic>ticks, appear to be distinct genotypes that may not belong to the species<jats:italic>R. rickettsii<\/jats:italic>. We were also able to differentiate 36 historical isolates of<jats:italic>R. rickettsii<\/jats:italic>into three different phylogenetic clades containing seven different genotypes. This differentiation correlated well, but not perfectly, with the geographic origin and likely tick vectors associated with the isolates. The few apparent typing discrepancies found suggest that the molecular ecology of<jats:italic>R. rickettsii<\/jats:italic>needs more investigation.<\/jats:p>","DOI":"10.1128\/jcm.00367-07","type":"journal-article","created":{"date-parts":[[2007,6,7]],"date-time":"2007-06-07T00:55:06Z","timestamp":1181177706000},"page":"2545-2553","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Molecular Typing of Isolates of<i>Rickettsia rickettsii<\/i>by Use of DNA Sequencing of Variable Intergenic Regions"],"prefix":"10.1128","volume":"45","author":[{"given":"Sandor E.","family":"Karpathy","sequence":"first","affiliation":[{"name":"Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Dasch","sequence":"additional","affiliation":[{"name":"Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina E.","family":"Eremeeva","sequence":"additional","affiliation":[{"name":"Rickettsial Zoonoses Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.51.2.653-660.1986"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.44.3.559-564.1984"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"461","DOI":"10.4049\/jimmunol.70.5.461","volume":"70","year":"1953","unstructured":"Bell, E. 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