{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:00:03Z","timestamp":1778778003601,"version":"3.51.4"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,9,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              To estimate the performance of anti-\n              <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n              IgA assay for the diagnosis of congenital toxoplasmosis, a retrospective monocenter study was conducted comparing serological results obtained in the framework of routine diagnosis workup. All infants born to mothers infected with\n              <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n              during pregnancy from 2010 to 2023 with at least 6 months of serological follow-up were included. Four hundred and eighty-three cases (1,171 sera) were included, of which 56 infants (11.6%) were infected. Twenty out of 60 infants (33.3%) with positive IgA were not infected. The sensitivity to detect IgA antibodies in infected neonates was 71.4%. The specificity was 97.3%. The mean time to detect IgA was 7.7 \u00b1 13.0 days in infected neonates. Anti-\n              <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n              IgA was the earliest positive serological test in only two cases (5.0%) but turned negative at 1 month in the absence of specific treatment, suggesting non-specific detection. IgA was associated with anti-\n              <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n              IgM, neosynthetized IgG or IgM on comparative Western blotting (WB), or both IgM and neosynthetized IgG or IgM on WB, in 12 cases (30.0%), 2 cases (5.0%), and 19 cases (47.5%), respectively. Sixteen infants with no IgA after birth were diagnosed via neosynthetized IgG or IgM on comparative WB and\/or IgM (\n              <jats:italic toggle=\"yes\">n<\/jats:italic>\n              = 10), or via PCR on amniotic fluid (\n              <jats:italic toggle=\"yes\">n<\/jats:italic>\n              = 5), or persistent IgG (\n              <jats:italic toggle=\"yes\">n<\/jats:italic>\n              = 1). Our study suggests that anti-\n              <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n              IgA is not a critical serological parameter for the diagnosis of congenital toxoplasmosis, and the recent withdrawal of commercialized reference anti-\n              <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n              IgA assays should not affect patient care.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                This study will help clinical microbiologists estimate the impact of the withdrawal of anti-\n                <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n                IgA reference assays for the diagnosis of congenital toxoplasmosis and will contribute to actualize the recommendations for laboratory diagnosis.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/jcm.00379-25","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T13:27:36Z","timestamp":1754918856000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Will we miss specific IgA detection for the diagnosis of congenital toxoplasmosis? A French retrospective monocenter study on 483 infants"],"prefix":"10.1128","volume":"63","author":[{"given":"Lya","family":"Hamet","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes","place":["Rennes, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Guegan","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes","place":["Rennes, France"]},{"name":"Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9 Environnement Travail)","place":["Rennes, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sorya","family":"Belaz","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes","place":["Rennes, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Pierre","family":"Gangneux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes","place":["Rennes, France"]},{"name":"Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9 Environnement Travail)","place":["Rennes, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4493-596X","authenticated-orcid":true,"given":"Florence","family":"Robert-Gangneux","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes","place":["Rennes, France"]},{"name":"Univ Rennes, CHU Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9 Environnement Travail)","place":["Rennes, France"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05013-11"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005222"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/590149"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jogoh.2021.102300"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit032"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02358-14"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181b20ed1"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00487-16"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-003-0887-5"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01222-23"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01368-20"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004310051168"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.45.7.605"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(99)70090-9"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.2893-2898.1999"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(00)00300-6"},{"key":"e_1_3_4_19_2","unstructured":"Dupouy-Camet J Bougnoux ME Souza S Thulliez P Dommergues M Mandelbrot L Ancelle T Tourte-Schaefer C Benarous R. 1992. 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