{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:15:10Z","timestamp":1793060110731,"version":"4.5.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2012,5,1]],"date-time":"2012-05-01T00:00:00Z","timestamp":1335830400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A PCR-based assay was developed to discriminate the classical, El Tor, and Haitian types of\n            <jats:italic>ctxB<\/jats:italic>\n            alleles. Our retrospective study using this newly developed PCR showed that Haitian\n            <jats:italic>ctxB<\/jats:italic>\n            first appeared in Kolkata during April 2006, and 93.3% of strains isolated during 2011 carried the new allele. Dendrogram analysis showed a pulsed-field gel electrophoresis (PFGE) pattern of the new variant strains isolated recently that was distinct from the PFGE pattern of the strains carrying classical\n            <jats:italic>ctxB<\/jats:italic>\n            that closely matched the 2006 to 2007 variant strains.\n          <\/jats:p>","DOI":"10.1128\/jcm.00387-12","type":"journal-article","created":{"date-parts":[[2012,2,23]],"date-time":"2012-02-23T01:41:32Z","timestamp":1329961292000},"page":"1733-1736","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Development and Evaluation of a PCR Assay for Tracking the Emergence and Dissemination of Haitian Variant\n            <i>ctxB<\/i>\n            in Vibrio cholerae O1 Strains Isolated from Kolkata, India"],"prefix":"10.1128","volume":"50","author":[{"given":"Arindam","family":"Naha","sequence":"first","affiliation":[{"name":"National Institute of Cholera and Enteric Diseases, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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