{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T11:48:37Z","timestamp":1778759317947,"version":"3.51.4"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A rapid molecular-based assay for the detection of the\n            <jats:named-content content-type=\"genus-species\">Candida albicans FKS1<\/jats:named-content>\n            gene mutations responsible for resistance to echinocandin drugs was designed and evaluated. The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most commonly described resistance mechanism, including\n            <jats:italic>FKS1<\/jats:italic>\n            hot spot 1 and hot spot 2 mutations. The performance and specificity of the assay was evaluated using a double-blinded panel of 50\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            strains. The assay showed a sensitivity of 96% and was able to detect all homozygous mutants included in the collection of strains, demonstrating that it is a robust, quick, and labor-saving method that is suitable for a routine clinical diagnostic laboratory.\n          <\/jats:p>","DOI":"10.1128\/jcm.00398-15","type":"journal-article","created":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T03:01:30Z","timestamp":1429153290000},"page":"2037-2041","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Quick Detection of\n            <i>FKS1<\/i>\n            Mutations Responsible for Clinical Echinocandin Resistance in Candida albicans"],"prefix":"10.1128","volume":"53","author":[{"given":"Catiana","family":"Dudiuk","sequence":"first","affiliation":[{"name":"Laboratorio de Micolog\u00eda y Diagn\u00f3stico Molecular, C\u00e1tedra de Parasitolog\u00eda y Micolog\u00eda, Facultad de Bioqu\u00edmica y Ciencias 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