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In children, typical enteropathogenic\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    (EPEC) is a common cause of diarrhea and is associated with a higher hazard of death. Typical EPEC infection is rare in animals and poorly reproduced in experimental animal models. In contrast, atypical EPEC (aEPEC) infection is common in both children and animals, but its role in diarrhea is uncertain. Mortality in kittens is often attributed to diarrhea, and we previously identified enteroadherent EPEC in the intestines of deceased kittens. The purpose of this study was to determine the prevalence and type of EPEC in kittens and whether infection was associated with diarrhea, diarrhea-related mortality, gastrointestinal pathology, or other risk factors. Kittens with and without diarrhea were obtained from two shelter facilities and determined to shed atypical EPEC at a culture-based prevalence of 18%. In contrast, quantitative PCR detected the presence of the gene for intimin (\n                    <jats:italic>eae<\/jats:italic>\n                    ) in feces from 42% of kittens. aEPEC was isolated from kittens with and without diarrhea. However, kittens with diarrhea harbored significantly larger quantities of aEPEC than kittens without diarrhea. Kittens with aEPEC had a significantly greater severity of small intestinal and colonic lesions and were significantly more likely to have required subcutaneous fluid administration. These findings identify aEPEC to be prevalent in kittens and a significant primary or contributing cause of intestinal inflammation, diarrhea, dehydration, and associated mortality in kittens.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00403-17","type":"journal-article","created":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T20:30:29Z","timestamp":1498681829000},"page":"2719-2735","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Association of Atypical Enteropathogenic Escherichia coli with Diarrhea and Related Mortality in Kittens"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3963-4695","authenticated-orcid":false,"given":"Victoria E.","family":"Watson","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan E.","family":"Jacob","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Flowers","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra J.","family":"Strong","sequence":"additional","affiliation":[{"name":"Wake County Animal Center, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitrita","family":"DebRoy","sequence":"additional","affiliation":[{"name":"E. coli Reference Center, Pennsylvania State University, University Park, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody L.","family":"Gookin","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60560-1"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60549-1"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001921"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32834a8b8b"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01664.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.01.015"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45719-0"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1204.051112"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000195623.57945.87"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.045443-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/648069"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.151.17.497"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1207\/s15327604jaws0704_1"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2014-8557"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.225.1399"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2005.10.003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00481-13"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.11.012"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1863-2378.2008.01190.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(00)01153-0"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.07.007"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098588702400407"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1959.03010320047011"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.20.7839"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1375-1377.1995"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(93)90082-I"},{"key":"e_1_3_3_29_2","first-page":"285","article-title":"Escherichia coli as a pathogen in dogs and cats","volume":"30","author":"Beutin L","year":"1999","unstructured":"Beutin L . 1999. 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