{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:58:03Z","timestamp":1761058683679},"reference-count":11,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infections with\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            are often asymptomatic. Liquid-based Pap (L-Pap) screening may provide samples for testing by commercial assays. Women attending a health clinic or a street youth clinic had a PreservCyt ThinPrep sample and a cervical swab (CS) collected. The L-Pap sample was tested for cytopathology; then 1 ml was transferred to an L-Pap specimen transfer tube for testing by the Gen-Probe APTIMA assays (APTIMA Combo 2 [AC2], APTIMA\n            <jats:italic>C. trachomatis<\/jats:italic>\n            [ACT], and APTIMA\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            [AGC]). The residual L-Pap sample was tested for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            using Roche AMPLICOR (AMP) and Becton Dickinson ProbeTec (PT). The CS was tested by AC2. A patient was considered infected if two specimens were positive or if a single specimen was positive in two tests. The prevalence of infection was 10% (29\/290) for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and 2.4% (7\/290) for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . Most of the positive patients had specimens that were reactive in all assays (20\/29 for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            ; 6\/7 for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            ). Four patients had double infections. The sensitivities and specificities of the various tests for the specimens tested were as follows. For\n            <jats:italic>C. trachomatis<\/jats:italic>\n            on L-Pap, sensitivity and specificity were 100 and 98.1%, respectively, for ACT, 93.1 and 98.8% for AC2, 86.2 and 91.2% for AMP, and 72.4 and 92.7% for PT. For\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            on L-Pap, sensitivity and specificity were 100% for both AGC and AC2, 85.7 and 100% for AMP, and 85.7 and 100% for PT. For AC2 with CSs, sensitivity and specificity were 93.1 and 98.5%, respectively, for\n            <jats:italic>C. trachomatis<\/jats:italic>\n            , and both were 100% for\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . There were significant differences in sensitivity and specificity (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). The APTIMA assays were more sensitive and specific than AMP or PT for detecting women's\n            <jats:italic>C. trachomatis<\/jats:italic>\n            and\/or\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            infections by testing ThinPrep samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.00405-07","type":"journal-article","created":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T00:54:28Z","timestamp":1180572868000},"page":"2355-2358","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Abilities of APTIMA, AMPLICOR, and ProbeTec Assays To Detect\n            <i>Chlamydia trachomatis<\/i>\n            and\n            <i>Neisseria gonorrhoeae<\/i>\n            in PreservCyt ThinPrep Liquid-Based Pap Samples"],"prefix":"10.1128","volume":"45","author":[{"given":"M.","family":"Chernesky","sequence":"first","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Jang","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Portillo","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Chong","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Smieja","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Luinstra","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Petrich","sequence":"additional","affiliation":[{"name":"St. Joseph's Healthcare\/McMaster University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"MacRitchie","sequence":"additional","affiliation":[{"name":"Hamilton Community Health Centre, Hamilton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Ewert","sequence":"additional","affiliation":[{"name":"Evergreen Health Centre"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Hayhoe","sequence":"additional","affiliation":[{"name":"Evergreen Health Centre"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sarabia","sequence":"additional","affiliation":[{"name":"MDS Laboratories, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Thompson","sequence":"additional","affiliation":[{"name":"MDS Laboratories, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.5.1749-1754.2002"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.400-405.2006"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.1084-1086.2006"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/dc.20325"},{"key":"e_1_3_1_6_2","first-page":"451","year":"1999","unstructured":"Hook, E. W., III, and H. H. Handsfield. 1999. Gonococcal infections in the adult, p. 451-466. In K. K. Holmes, P.-A. M\u00e5rdh, F. P. Sparling, et al. (ed.), Sexually transmitted diseases, 3rd ed. McGraw-Hill Companies, Inc., New York, NY.","journal-title":"Sexually transmitted diseases"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2003.008359"},{"key":"e_1_3_1_8_2","first-page":"237","volume":"46","year":"2001","unstructured":"Inhorn, S. L., P. J. Wand, T. C. Wright, K. D. Hatch, J. Hallum, and B. B. Lentrichia. 2001. Chlamydia trachomatis and pap testing from a single, fluid-based sample: a multicentre study. J. Reprod. Med.46:237-242.","journal-title":"J. Reprod. Med."},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1507-1511.2003"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.5858\/2003-127-959-HPDTAA"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000190092.59482.96"},{"key":"e_1_3_1_12_2","first-page":"407","year":"1999","unstructured":"Stamm, W. E. 1999. Chlamydia trachomatis infections of the adult, p. 407-422. In K. K. Holmes, P.-A. M\u00e5rdh, F. P. Sparling, et al. (ed.), Sexually transmitted diseases, 3rd ed. McGraw-Hill Companies, Inc., New York, NY.","journal-title":"Sexually transmitted diseases"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00405-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00405-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:54:55Z","timestamp":1646459695000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00405-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":11,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1128\/JCM.00405-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00405-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]},"assertion":[{"value":"2007-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}