{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:44:02Z","timestamp":1785437042090,"version":"3.56.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,10,8]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Corynebacterium jeikeium<\/jats:italic>\n is an opportunistic human pathogen with high genomic diversity. In the past, multi-drug resistance has been considered a hallmark of\n C. jeikeium<\/jats:italic>\n . However, some strains of\n C. jeikeium<\/jats:italic>\n are fully susceptible and thus, their reclassification would avoid the empirical therapy with glycopeptides. One hundred eighty-six clinical isolates and four reference isolates identified as\n C. jeikeium<\/jats:italic>\n between 1994\u20131999 and 2012\u20132019, respectively, were analyzed by matrix-assisted laser desorption\/ionization time of flight mass spectrometry (MALDI-TOF MS) and 16S ribosomal RNA (16S rRNA) gene sequence analysis. A total of 153 confirmed\n C. jeikeium<\/jats:italic>\n isolates was further analyzed by whole genome sequencing (WGS) and by disk diffusion antimicrobial susceptibility testing (AST). WGS and subsequent core genome single nucleotide polymorphism-based phylogenetic analysis segregated the isolates into seven phylogenetic clusters. The average nucleotide identity between the clusters was\n <<\/jats:underline>\n 95%, qualifying them as separate\n C. jeikeium<\/jats:italic>\n genomospecies. Overall, AST profiles of all 153 isolates were heterogeneous for six of the 16 antibiotics tested. Notably, genomospecies 6 and 7 displayed mainly comparably small inhibition zones. For the other 10 antibiotics, all genomospecies displayed either small or large inhibition zones, respectively. Our study of\n C. jeikeium<\/jats:italic>\n clinical isolates revealed a relatively complex phylogenetic structure. As soon as clear phenotypic traits can be attributed to the seven genomospecies by routine diagnostic tests, genomospecies encompassing mainly antibiotic-susceptible strains should be reclassified to fine-tune potential treatment strategies.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Corynebacterium jeikeium<\/jats:italic>\n isolates typically display multi-drug resistance (MDR), frequently triggering empirical therapies with glycopeptides. However, some strains are susceptible to a broad range of antibiotics. In our study, analysis of a large collection of\n C. jeikeium<\/jats:italic>\n by whole genome sequencing (WGS) and antibiotic resistance testing revealed genomic diversity, and some correlations between MDR phenotypes and specific genomospecies. While WGS is not yet a routine method, identification of MDR genomospecies may confirm the need for glycopeptide therapy. Identification of a less-resistant genomospecies would have even a higher clinical impact, as unnecessary therapies with glycopeptides are avoided, thereby reducing the selection for vancomycin-resistant enterococci. In the future, databases of diagnostic tools, e.g., MALDI-TOF, may be expanded to allow for the differentiation of genomospecies. Furthermore, rational taxonomic reclassification of some\n C. jeikeium<\/jats:italic>\n genomospecies would help clinicians to fine-tune potential treatment strategies.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/jcm.00418-25","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T13:01:49Z","timestamp":1756904509000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Antibiotic resistance profiles of seven genomospecies of\n Corynebacterium jeikeium<\/i>\n analyzed by whole genome sequencing"],"prefix":"10.1128","volume":"63","author":[{"given":"Michaela","family":"Fischer-Wellenborn","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02crff812","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Medical Microbiology, University of Zurich","place":["Z\u00fcrich, 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