{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:02:26Z","timestamp":1793149346611,"version":"4.5.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2023,10,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rapid identification of the causative pathogens of central nervous system infections is essential for providing appropriate management and improving patient outcomes. The performance of QIAstat-Dx Meningitis\/Encephalitis (ME) Panel\u2014a multiplex PCR testing platform\u2014in detecting pathogens implicated in meningitis and\/or encephalitis was evaluated using BioFire FilmArray ME Panel as a comparator method. This multicenter study analyzed 585 retrospective residual cerebrospinal fluid specimens and 367 contrived specimens. The QIAstat-Dx ME Panel showed positive percent agreement (PPA) values of 100% for\n                    <jats:italic>Neisseria meningitidis<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    K1,\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    , and\n                    <jats:italic>Cryptococcus gattii<\/jats:italic>\n                    \/\n                    <jats:italic>neoformans<\/jats:italic>\n                    on clinical samples compared to the BioFire FilmArray ME Panel. The PPA values observed for\n                    <jats:italic>Haemophilus influenzae<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    were 80% and 88.24%, respectively. Negative percent agreement (NPA) values were &gt;99.0% for each of the six bacterial targets and one fungal target tested with clinical samples. One viral target, herpes simplex virus 1, exhibited a PPA value of 100.0%, while the remaining viral targets\u2014human parechovirus, herpes simplex virus 2, human herpes virus 6, and varicella zoster virus\u2014were &gt;90.0%, with the exception of enterovirus, which had a PPA value of 77.8%. The QIAstat-Dx ME Panel detected five true-positive and four true-negative cases compared to BioFire FilmArray ME Panel. The NPA values for all viral pathogens were &gt;99.0%. Overall, the QIAstat-Dx ME Panel showed comparable performance to the BioFire FilmArray ME Panel as a rapid diagnostic tool for community-acquired meningitis and encephalitis.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00426-23","type":"journal-article","created":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T09:00:48Z","timestamp":1694595648000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Evaluation of the QIAstat-Dx Meningitis\/Encephalitis Panel, a multiplex PCR platform for the detection of community-acquired meningoencephalitis"],"prefix":"10.1128","volume":"61","author":[{"given":"Thomas","family":"Sundelin","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Herlev and Gentofte Hospital , Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Bialas","sequence":"additional","affiliation":[{"name":"Labor Berlin-Charite Vivantes Services GmbH , Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juana","family":"de Diego","sequence":"additional","affiliation":[{"name":"Labor Berlin-Charite Vivantes Services GmbH , Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Hermanowski","sequence":"additional","affiliation":[{"name":"Labor Berlin-Charite Vivantes Services GmbH , Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrik","family":"Leibhan","sequence":"additional","affiliation":[{"name":"Labor Berlin-Charite Vivantes Services GmbH , Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a","family":"Ponderand","sequence":"additional","affiliation":[{"name":"Laboratoire de bact\u00e9riologie, CHU Grenoble Alpes , La Tronche, France"},{"name":"Universit\u00e9 Grenoble Alpes, CEA, CNRS, IBS , Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4566-7154","authenticated-orcid":false,"given":"Marti","family":"Juanola-Falgarona","sequence":"additional","affiliation":[{"name":"STAT-Dx Life S.L. (a QIAGEN Company), Carrer de Baldiri Reixac , Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Jones","sequence":"additional","affiliation":[{"name":"QIAGEN Ltd , Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9433-2222","authenticated-orcid":false,"given":"Melisa","family":"Rey","sequence":"additional","affiliation":[{"name":"STAT-Dx Life S.L. (a QIAGEN Company), Carrer de Baldiri Reixac , Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Johnson","sequence":"additional","affiliation":[{"name":"QIAGEN Ltd , Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Pareja","sequence":"additional","affiliation":[{"name":"STAT-Dx Life S.L. (a QIAGEN Company), Carrer de Baldiri Reixac , Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvan","family":"Caspar","sequence":"additional","affiliation":[{"name":"Laboratoire de bact\u00e9riologie, CHU Grenoble Alpes , La Tronche, France"},{"name":"Universit\u00e9 Grenoble Alpes, CEA, CNRS, IBS , Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30387-9"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2016.01.007"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00024-17"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/589747"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.01.007"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit458"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.74"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00070-09"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00730-16"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410340404"},{"key":"e_1_3_4_12_2","volume-title":"Qiastat-DX meningitis\/encephalitis (ME) panel instructions for use (Handbook)","author":"QIAGEN GmbH","year":"2022","unstructured":"QIAGEN GmbH . 2022. Qiastat-DX meningitis\/encephalitis (ME) panel instructions for use (Handbook). QIAGEN GmbH, Hilden, Germany."},{"key":"e_1_3_4_13_2","volume-title":"Biofire Filmarray ME panel CE IVD instruction booklet","author":"BioFire Diagnostics","year":"2021","unstructured":"BioFire Diagnostics . 2021. Biofire Filmarray ME panel CE IVD instruction booklet. BioFire Diagnostics, LLC, Salt Lake City, UT. https:\/\/www.biofiredx.qarad.eifu.online\/ITI\/US\/en\/all?keycode=ITI0035."},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.11.016"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/648419"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.05.017"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofx242"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myz064"},{"key":"e_1_3_4_19_2","unstructured":"Panel on Opportunistic Infections in Adults and Adolescents with HIV\n. n.d. Guidelines for the prevention and treatment of opportunistic infections in adults and adolescents with HIV:Recommendations from the centers for disease control and prevention the National Institutes of health and the HIV medicine Association of the infectious diseases society of America. Available from: http:\/\/aidsinfo.nih.gov\/contentfiles\/lvguidelines\/adult_oi.pdf"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/lam.13375"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2020-0380-OA"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.9.3.508-514.2002"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01927-17"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2005.07.005"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.12.006"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00426-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00426-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T09:01:13Z","timestamp":1698138073000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00426-23"}},"subtitle":[],"editor":[{"given":"Erin","family":"McElvania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,10,24]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10,24]]}},"alternative-id":["10.1128\/jcm.00426-23"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00426-23","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,24]]},"assertion":[{"value":"2023-04-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00426-23"}}