{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:23:20Z","timestamp":1792610600202,"version":"4.5.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2023,10,24]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cytomegalovirus (CMV) is a significant cause of morbidity and mortality among immunocompromised hosts, including transplant recipients. Antiviral prophylaxis or treatment is used to reduce the incidence of CMV disease in this patient population; however, there is concern about increasing antiviral resistance. Detection of antiviral resistance in CMV was traditionally accomplished using Sanger sequencing of\n            <jats:italic>UL54<\/jats:italic>\n            and\n            <jats:italic>UL97<\/jats:italic>\n            genes, in which specific mutations may result in reduced antiviral activity. In this study, a novel next-generation sequencing (NGS) method was developed and validated to detect mutations in\n            <jats:italic>UL54<\/jats:italic>\n            \/\n            <jats:italic>UL97<\/jats:italic>\n            associated with antiviral resistance. Plasma samples (\n            <jats:italic>n<\/jats:italic>\n            = 27) submitted for antiviral resistance testing by Sanger sequencing were also analyzed using the NGS method. When compared to Sanger sequencing, the NGS assay demonstrated 100% (27\/27) overall agreement for determining antiviral resistance\/susceptibility and 88% (22\/25) agreement at the level of resistance-associated mutations. The limit of detection of the NGS method was determined to be 500 IU\/mL, and the lower threshold for detecting mutations associated with resistance was established at 15%. The NGS assay represents a novel laboratory tool that assists healthcare providers in treating patients who are infected with CMV harboring resistance-associated mutations and who may benefit from tailored antiviral therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.00429-23","type":"journal-article","created":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T13:06:22Z","timestamp":1695733582000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Use of next-generation sequencing to detect mutations associated with antiviral drug resistance in cytomegalovirus"],"prefix":"10.1128","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-3406","authenticated-orcid":false,"given":"Nicholas T.","family":"Streck","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Espy","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Ferber","sequence":"additional","affiliation":[{"name":"Division of Laboratory Genetics and Genomics, Mayo Clinic , Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric W.","family":"Klee","sequence":"additional","affiliation":[{"name":"Division of Biomedical Statistics and Informatics, Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymund R.","family":"Razonable","sequence":"additional","affiliation":[{"name":"Division of Public Health, Infectious Diseases and Occupational Medicine, Mayo Clinic , Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitri","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"ABL Diagnostics , Woippy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chalom","family":"Sayada","sequence":"additional","affiliation":[{"name":"ABL Diagnostics , Woippy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip R.","family":"Heaton","sequence":"additional","affiliation":[{"name":"Health Partners Medical Laboratory and Pathology Services , Bloomington, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunwen","family":"Chou","sequence":"additional","affiliation":[{"name":"Research Service, Department of Veterans Affairs Medical Center , Portland, Oregon, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2537-7165","authenticated-orcid":false,"given":"Matthew J.","family":"Binnicker","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/652438"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81891-0"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000000160"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3026(15)00289-6"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-11-679639"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000409"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0012.2007.00761.x"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12241"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000541"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1873"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2020.104711"},{"key":"e_1_3_1_13_2","first-page":"900","volume-title":"The third international consensus guidelines on the management of cytomegalovirus in solid-organ transplantation.","author":"Kotton CN","year":"2018","unstructured":"Kotton CN , Kumar D , Caliendo AM , Huprikar S , Chou S , Danziger-Isakov L , Humar A . 2018. The transplantation society International CMVCG, p 900\u2013931. In The third international consensus guidelines on the management of cytomegalovirus in solid-organ transplantation."},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00009-10"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2021.105123"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0219701"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00429-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00429-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T13:01:55Z","timestamp":1698152515000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00429-23"}},"subtitle":[],"editor":[{"given":"Yi-Wei","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,10,24]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10,24]]}},"alternative-id":["10.1128\/jcm.00429-23"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00429-23","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,24]]},"assertion":[{"value":"2023-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00429-23"}}