{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T07:03:34Z","timestamp":1778051014235,"version":"3.51.4"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We describe the first reported case of\n            <jats:named-content content-type=\"genus-species\">Ureaplasma parvum<\/jats:named-content>\n            prosthetic joint infection (PJI) detected by PCR.\n            <jats:named-content content-type=\"genus-species\">Ureaplasma<\/jats:named-content>\n            species do not possess a cell wall and are usually associated with colonization and infection of mucosal surfaces (not prosthetic material).\n            <jats:named-content content-type=\"genus-species\">U. parvum<\/jats:named-content>\n            is a relatively new species name for certain serovars of\n            <jats:named-content content-type=\"genus-species\">Ureaplasma urealyticum<\/jats:named-content>\n            , and PCR is useful for species determination. Our patient presented with late infection of his right total knee arthroplasty. Intraoperative fluid and tissue cultures and pre- and postoperative synovial fluid cultures were all negative. To discern the pathogen, we employed PCR coupled with electrospray ionization mass spectrometry (PCR\/ESI-MS). Our patient's failure to respond to empirical antimicrobial treatment and our previous experience with PCR\/ESI-MS in culture-negative cases of infection prompted us to use this approach over other diagnostic modalities. PCR\/ESI-MS detected\n            <jats:named-content content-type=\"genus-species\">U. parvum<\/jats:named-content>\n            in all samples.\n            <jats:named-content content-type=\"genus-species\">U. parvum<\/jats:named-content>\n            -specific PCR testing was performed on all synovial fluid samples to confirm the\n            <jats:named-content content-type=\"genus-species\">U. parvum<\/jats:named-content>\n            detection.\n          <\/jats:p>","DOI":"10.1128\/jcm.00432-14","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T02:11:36Z","timestamp":1395886296000},"page":"2248-2250","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Ureaplasma parvum Prosthetic Joint Infection Detected by PCR"],"prefix":"10.1128","volume":"52","author":[{"given":"John J.","family":"Farrell","sequence":"first","affiliation":[{"name":"University of Illinois School of Medicine, Department of Medicine, Peoria, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua A.","family":"Larson","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, St. Francis Medical Center, Peoria, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey W.","family":"Akeson","sequence":"additional","affiliation":[{"name":"University of Illinois School of Medicine, Department of Orthopedic Surgery, Peoria, Illinois, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin S.","family":"Lowery","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan A.","family":"Rounds","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rangarajan","family":"Sampath","sequence":"additional","affiliation":[{"name":"Ibis Biosciences, Carlsbad, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[{"name":"Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Research Service, Cleveland, Ohio, USA"},{"name":"Case Western Reserve University, Departments of Medicine, Pharmacology and Molecular Microbiology, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Divisions of Clinical Microbiology and Infectious Diseases, Mayo Clinic, Rochester, Minnesota, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2010.02.2253"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2010.01.019"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2009.10.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.otsr.2009.10.010"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00834-12"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.08.005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/168742"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02352-12"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066349"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00936-10"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-999-4_7"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis803"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00432-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00432-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:58:50Z","timestamp":1646481530000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00432-14"}},"subtitle":[],"editor":[{"given":"K. C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":12,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1128\/JCM.00432-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00432-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]},"assertion":[{"value":"2014-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}