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Molecular analysis by PCR and sequencing represents the only reliable tool to date to identify these parasites to the species level. Due to the recent spread of human diphyllobothriosis in several countries, a correct diagnosis has become crucial to better understand the distribution and the life cycle of human-infecting species as well as to prevent the introduction of parasites to disease-free water systems. Nevertheless, PCR and sequencing, although highly precise, are too complicated, long, and expensive to be employed in medical laboratories for routine diagnostics. In the present study we optimized a cheap and rapid molecular test for the differential identification of the most common<jats:italic>Diphyllobothrium<\/jats:italic>species infecting humans (<jats:italic>D. latum<\/jats:italic>,<jats:italic>D. dendriticum<\/jats:italic>,<jats:italic>D. nihonkaiense<\/jats:italic>, and<jats:italic>D. pacificum<\/jats:italic>), based on a multiplex PCR with the cytochrome<jats:italic>c<\/jats:italic>oxidase subunit 1 gene of mitochondrial DNA.<\/jats:p>","DOI":"10.1128\/jcm.00445-10","type":"journal-article","created":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T02:37:21Z","timestamp":1277951841000},"page":"3111-3116","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["Multiplex PCR for Differential Identification of Broad Tapeworms (<i>Cestoda<\/i>:<i>Diphyllobothrium<\/i>) Infecting Humans"],"prefix":"10.1128","volume":"48","author":[{"given":"Barbara","family":"Wicht","sequence":"first","affiliation":[{"name":"Istituto Cantonale di Microbiologia, Bellinzona, Switzerland"},{"name":"Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, C\u030ceske\u0301 Bude\u030cjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Yanagida","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toma\u0301s\u030c","family":"Scholz","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, C\u030ceske\u0301 Bude\u030cjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Asahikawa Medical College, Asahikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Jime\u0301nez","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Universidad Peruana Cayetano Heredia, and Cysticercosis Unit, Instituto de Ciencias Neurolo\u0301gicas and Instituto Peruano de Parasitologi\u0301a Clinica y Experimental, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Brabec","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, C\u030ceske\u0301 Bude\u030cjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00006946"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-6409.2006.00250.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.013"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00009742"},{"key":"e_1_3_2_7_2","first-page":"91","volume":"37","year":"1988","unstructured":"Fukumoto, S., S. 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