{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:48:18Z","timestamp":1790160498838,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and LGV reference strains and did not cross-react with the DNA from 26 strains representing the bacterial pathogens and commensals of the oropharynx, genital tract, and rectum. The NRT-PCR had a 95% probability of detection at four genome copies (confidence interval, three to six copies) of\n            <jats:italic>C. trachomatis<\/jats:italic>\n            per reaction. One hundred cervical and urethral swab specimens containing\n            <jats:italic>C. trachomatis<\/jats:italic>\n            DNA from 63 women and 37 men were used to validate the method. The results from the NRT-PCR and the DNA sequencing of amplicons generated from the\n            <jats:italic>omp1<\/jats:italic>\n            gene showed 100% correlation for these samples. The assay also identified the LGV-II genotype in 24 of 48 rectal swab specimens containing\n            <jats:italic>C. trachomatis<\/jats:italic>\n            DNA that were obtained from men having sex with men. The Sexually Transmitted Bacteria Reference Laboratory, London, independently confirmed these results using group-specific LGV real-time PCR and restriction fragment length polymorphism analysis. Compared with the NRT-PCR, non-NRT-PCR was found to be less sensitive: it typed\n            <jats:italic>C. trachomatis<\/jats:italic>\n            DNA in only 80% of the genital samples and 90% of the rectal swab samples. This is the first successful demonstration of the use of real-time PCR for the genotype-specific typing of\n            <jats:italic>C. trachomatis<\/jats:italic>\n            strains that cause sexually transmitted diseases.\n          <\/jats:p>","DOI":"10.1128\/jcm.00451-07","type":"journal-article","created":{"date-parts":[[2007,6,14]],"date-time":"2007-06-14T04:04:39Z","timestamp":1181793879000},"page":"2649-2653","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Development of Real-Time PCR Assays for Genotyping of\n            <i>Chlamydia trachomatis<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Hamid","family":"Jalal","sequence":"first","affiliation":[{"name":"Clinical Microbiology &amp; Public Health Laboratory, Box 236, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Stephen","sequence":"additional","affiliation":[{"name":"Clinical Microbiology &amp; Public Health Laboratory, Box 236, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QW, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Alexander","sequence":"additional","affiliation":[{"name":"Sexually Transmitted Bacteria Reference Laboratory, Centre for Infection, 61 Colindale Avenue, London NW9 5HT, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Carne","sequence":"additional","affiliation":[{"name":"Department of Genitourinary Medicine, Clinic 1A, Box 38, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Sonnex","sequence":"additional","affiliation":[{"name":"Department of Genitourinary Medicine, Clinic 1A, Box 38, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.77.6.419"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.5.1103"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2006.02.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2005.015362"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.206-213.2006"},{"key":"e_1_3_2_7_2","unstructured":"Int. 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