{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:50:55Z","timestamp":1761519055445},"reference-count":34,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Transcription-mediated amplification (TMA) enhances detection of\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            from rectal and pharyngeal sources. The utility of TMA for detection of\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            has recently been described. We report on the performance of TMA for detection of sexually transmitted infection (STI) agents from extraurogenital sources, with a focus on\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            . Within a 21-month interval, 1,314 consecutive male patient encounters at an STI clinic resulted in collection of 2,408 specimens for\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            TMA screening. A total of 471 encounters were managed with a single specimen collection (94.9% urine), with 12.7% positive for at least one STI agent. This detection percentage increased to 14.4% with collection of specimens from two sources and to 20.3% with collection from three sources (\n            <jats:italic>P<\/jats:italic>\n            = 0.03 versus single-source sampling). A total of 44.4% of encounters were managed by collection of urine and pharyngeal specimens and 19.1% by the addition of a third (rectal) collection. While procurement of urine and rectal specimens resulted in greater detection of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            (6.1% and 11.3% rates, respectively) than of other STI agents, 858 pharyngeal specimens yielded a 2.9%\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            detection rate compared with 2.1% for\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            and 1.6% for\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            . All\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            pharyngeal detections were confirmed by TMA-based alternative target testing. A total of 38.1% of\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            -positive pharyngeal specimens were derived from symptomatic patient encounters. A total of 85.7% of males with\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            -positive pharyngeal collections indicated strictly heterosexual preference. Additional specimen source sampling is necessary to make STI screening comprehensive. Incorporation of extraurogenital sources into assessment for\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            detection may identify additional symptomatic and asymptomatic male STI carriers.\n          <\/jats:p>","DOI":"10.1128\/jcm.00455-13","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T06:34:49Z","timestamp":1365057289000},"page":"1855-1860","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Retrospective Assessment of Transcription-Mediated Amplification-Based Screening for Trichomonas vaginalis in Male Sexually Transmitted Infection Clinic Patients"],"prefix":"10.1128","volume":"51","author":[{"given":"Erik","family":"Munson","sequence":"first","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin, USA"},{"name":"College of Health Sciences, University of Wisconsin\u2014Milwaukee, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Wenten","sequence":"additional","affiliation":[{"name":"STD Specialties Clinic, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Phipps","sequence":"additional","affiliation":[{"name":"STD Specialties Clinic, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Gremminger","sequence":"additional","affiliation":[{"name":"STD Specialties Clinic, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Kay","family":"Schuknecht","sequence":"additional","affiliation":[{"name":"STD Specialties Clinic, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Napierala","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deb","family":"Hamer","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin, USA"},{"name":"STD Specialties Clinic, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Olson","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald F.","family":"Schell","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, University of Wisconsin, Madison, Wisconsin, USA"},{"name":"Departments of Microbiology, University of Wisconsin, Madison, Wisconsin, USA"},{"name":"Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne 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