{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:28:17Z","timestamp":1790533697423,"version":"4.1.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated whether the Bruker Biotyper matrix-associated laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) system provides accurate species-level identifications of 147 isolates of aerobically growing Gram-positive rods (GPRs). The bacterial isolates included\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 74),\n            <jats:named-content content-type=\"genus-species\">Listeria<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 39),\n            <jats:named-content content-type=\"genus-species\">Kocuria<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 15),\n            <jats:named-content content-type=\"genus-species\">Rhodococcus<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 10),\n            <jats:named-content content-type=\"genus-species\">Gordonia<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 7), and\n            <jats:named-content content-type=\"genus-species\">Tsukamurella<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 2) species, which had all been identified by conventional methods, molecular methods, or both. In total, 89.7% of\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            , 80% of\n            <jats:named-content content-type=\"genus-species\">Rhodococcus<\/jats:named-content>\n            species, 26.7% of\n            <jats:named-content content-type=\"genus-species\">Kocuria<\/jats:named-content>\n            species, and 14.9% of\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            species (\n            <jats:italic>n<\/jats:italic>\n            = 11, all\n            <jats:named-content content-type=\"genus-species\">N. nova<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. otitidiscaviarum<\/jats:named-content>\n            ) were correctly identified to the species level (score values, \u22652.0). A clustering analysis of spectra generated by the Bruker Biotyper identified six clusters of\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            species, i.e., cluster 1 (\n            <jats:named-content content-type=\"genus-species\">N. cyriacigeorgica<\/jats:named-content>\n            ), cluster 2 (\n            <jats:named-content content-type=\"genus-species\">N. brasiliensis<\/jats:named-content>\n            ), cluster 3 (\n            <jats:named-content content-type=\"genus-species\">N. farcinica<\/jats:named-content>\n            ), cluster 4 (\n            <jats:named-content content-type=\"genus-species\">N. puris<\/jats:named-content>\n            ), cluster 5 (\n            <jats:named-content content-type=\"genus-species\">N. asiatica<\/jats:named-content>\n            ), and cluster 6 (\n            <jats:named-content content-type=\"genus-species\">N. beijingensis<\/jats:named-content>\n            ), based on the six peaks generated by ClinProTools with the genetic algorithm, i.e.,\n            <jats:italic>m\/z<\/jats:italic>\n            2,774.477 (cluster 1),\n            <jats:italic>m\/z<\/jats:italic>\n            5,389.792 (cluster 2),\n            <jats:italic>m\/z<\/jats:italic>\n            6,505.720 (cluster 3),\n            <jats:italic>m\/z<\/jats:italic>\n            5,428.795 (cluster 4),\n            <jats:italic>m\/z<\/jats:italic>\n            6,525.326 (cluster 5), and\n            <jats:italic>m\/z<\/jats:italic>\n            16,085.216 (cluster 6). Two clusters of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            spectra were also found according to the five peaks, i.e.,\n            <jats:italic>m\/z<\/jats:italic>\n            5,594.85,\n            <jats:italic>m\/z<\/jats:italic>\n            6,184.39, and\n            <jats:italic>m\/z<\/jats:italic>\n            11,187.31, for cluster 1 (serotype 1\/2a) and\n            <jats:italic>m\/z<\/jats:italic>\n            5,601.21 and\n            <jats:italic>m\/z<\/jats:italic>\n            11,199.33 for cluster 2 (serotypes 1\/2b and 4b). The Bruker Biotyper system was unable to accurately identify\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            (except for\n            <jats:named-content content-type=\"genus-species\">N. nova<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. otitidiscaviarum<\/jats:named-content>\n            ),\n            <jats:named-content content-type=\"genus-species\">Tsukamurella<\/jats:named-content>\n            , or\n            <jats:named-content content-type=\"genus-species\">Gordonia<\/jats:named-content>\n            species. Continuous expansion of the MALDI-TOF MS databases to include more GPRs is necessary.\n          <\/jats:p>","DOI":"10.1128\/jcm.00456-14","type":"journal-article","created":{"date-parts":[[2014,4,24]],"date-time":"2014-04-24T03:17:39Z","timestamp":1398309459000},"page":"2371-2379","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Bruker Biotyper Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry System for Identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria Species"],"prefix":"10.1128","volume":"52","author":[{"given":"Po-Ren","family":"Hsueh","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan"},{"name":"Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai-Fen","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan"},{"name":"Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-Hei","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih-Hua","family":"Teng","sequence":"additional","affiliation":[{"name":"Department of Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan, Taiwan"},{"name":"Bruker Taiwan Co., Ltd., Taipei, 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