{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T13:58:51Z","timestamp":1782482331017,"version":"3.54.5"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Brucellosis remains an important anthropozoonosis worldwide.\n            <jats:italic>Brucella<\/jats:italic>\n            species are genetically homogeneous, and thus, the typing of\n            <jats:italic>Brucella<\/jats:italic>\n            species for epidemiological purposes by conventional molecular typing methods has remained elusive. Although many methods could segregate isolates into the phylogenetically recognized taxa, limited within-species genetic diversity has been identified. Recently, multilocus variable-number tandem-repeat analysis (MLVA) was found to have a high degree of resolution when it was applied to collections of\n            <jats:italic>Brucella<\/jats:italic>\n            isolates from geographically widespread locations, and an assay comprising 16 such loci (MLVA-16) was proposed. This scheme includes eight minisatellite loci (panel 1) and eight microsatellites (panel 2, which is subdivided into panels 2A and 2B). The utility of MLVA-16 for the subtyping of human\n            <jats:italic>Brucella<\/jats:italic>\n            isolates from geographically restricted regions needs to be further evaluated, and genotyping databases with worldwide coverage must be progressively established. In the present study, MLVA-16 was applied to the typing of 42 human\n            <jats:italic>Brucella<\/jats:italic>\n            isolates obtained from 41 patients recovered from 2002 to 2006 at a tertiary-care center in Lebanon. All isolates were identified as\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            by MLVA-16 and were found to be closely related to\n            <jats:italic>B. melitensis<\/jats:italic>\n            isolates from neighboring countries in the Middle East when their genotypes were queried against those in the web-based Brucella2007 MLVA database (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"url\" xlink:href=\"http:\/\/mlva.u-psud.fr\/\">http:\/\/mlva.u-psud.fr\/<\/jats:ext-link>\n            ). Panel 2B, which comprised the most variable loci, displayed a very high discriminatory power, while panels 1 and 2A showed limited diversity. The most frequent genotype comprised seven isolates obtained over 7 weeks in 2002, demonstrating an outbreak from a common source. Two isolates obtained from one patient 5 months apart comprised another genotype, indicating relapsing disease. These findings confirm that MLVA-16 has a good discriminatory power for species determination, typing of\n            <jats:italic>B. melitensis<\/jats:italic>\n            isolates, and inferring their geographical origin. Abbreviated panel 2B could be used as a short-term epidemiological tool in a small region of endemicity.\n          <\/jats:p>","DOI":"10.1128\/jcm.00464-08","type":"journal-article","created":{"date-parts":[[2008,10,16]],"date-time":"2008-10-16T03:34:28Z","timestamp":1224128068000},"page":"3935-3940","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Evaluation of a Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Typing Human\n            <i>Brucella<\/i>\n            Isolates in a Region of Brucellosis Endemicity"],"prefix":"10.1128","volume":"46","author":[{"given":"Mireille M.","family":"Kattar","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine"},{"name":"World Health Organization Regional Collaborating Center on Human Brucellosis, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rola F.","family":"Jaafar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, American University of Beirut, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George F.","family":"Araj","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine"},{"name":"World Health Organization Regional Collaborating Center on Human Brucellosis, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Le Fle\u0300che","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Sud 11, CNRS, UMR8621, Institut de Ge\u0301ne\u0301tique et Microbiologie, Orsay 91405, France"},{"name":"Department of Analytical Microbiology, Centre d'Etudes du Bouchet, Vert le Petit 91710, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghassan M.","family":"Matar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, American University of Beirut, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Abi Rached","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine"},{"name":"World Health Organization Regional Collaborating Center on Human Brucellosis, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Khalife","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine"},{"name":"World Health Organization Regional Collaborating Center on Human Brucellosis, Beirut, Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Sud 11, CNRS, UMR8621, Institut de Ge\u0301ne\u0301tique et Microbiologie, Orsay 91405, France"},{"name":"DGA\/D4S-Mission pour la Recherche et l'Innovation Scientifique, Bagneux 92220, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.015"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1312.070527"},{"key":"e_1_3_2_4_2","first-page":"352","volume":"511","year":"2005","unstructured":"Al-Dahouk, S., R. 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