{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:20:23Z","timestamp":1774678823397,"version":"3.50.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI098713"],"award-info":[{"award-number":["R01AI098713"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Bacillus anthracis<\/jats:named-content>\n            is a tier 1 select agent with the potential to quickly cause severe disease. Rapid identification of this pathogen may accelerate treatment and reduce mortality in the event of a bioterrorism attack. We developed a rapid and sensitive assay to detect\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            bacteremia using a system that is suitable for point-of-care testing. A filter-based cartridge that included both sample processing and PCR amplification functions was loaded with all reagents needed for sample processing and multiplex nested PCR. The assay limit of detection (LOD) and dynamic range were determined by spiking\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            DNA into individual PCR mixtures and\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            CFU into human blood. One-milliliter blood samples were added to the filter-based detection cartridge and tested for\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            on a GeneXpert instrument. Assay specificity was determined by testing blood spiked with non-anthrax bacterial isolates or by testing blood samples drawn from patients with concurrent non-\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            bacteremia or nonbacteremic controls. The assay LODs were 5 genome equivalents per reaction and 10 CFU\/ml blood for both the\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            Sterne and V1B strains. There was a 6-log\n            <jats:sub>10<\/jats:sub>\n            dynamic range. Assay specificity was 100% for tests of non-\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            bacterial isolates and patient blood samples. Assay time was less than 90 min. This automated system suitable for point-of-care detection rapidly identifies\n            <jats:named-content content-type=\"genus-species\">B. anthracis<\/jats:named-content>\n            directly from blood with high sensitivity. This assay might lead to early detection and more rapid therapy in the event of a bioterrorism attack.\n          <\/jats:p>","DOI":"10.1128\/jcm.00466-17","type":"journal-article","created":{"date-parts":[[2017,7,27]],"date-time":"2017-07-27T00:40:26Z","timestamp":1501116026000},"page":"2964-2971","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Rapid Detection of Bacillus anthracis Bloodstream Infections by Use of a Novel Assay in the GeneXpert System"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5217-6142","authenticated-orcid":false,"given":"Padmapriya P.","family":"Banada","sequence":"first","affiliation":[{"name":"Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srinidhi","family":"Deshpande","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Russo","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Singleton","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darshini","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University Hospital, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhavana","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University Hospital, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele","family":"Burday","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University Hospital, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranie","family":"Koshy","sequence":"additional","affiliation":[{"name":"Blood Bank\/Transfusion Service, University Hospital, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University Hospital, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Jones","sequence":"additional","affiliation":[{"name":"Cepheid, Sunnyvale, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Gall","sequence":"additional","affiliation":[{"name":"Cepheid, Bothell, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Lokhov","sequence":"additional","affiliation":[{"name":"Cepheid, Bothell, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Kwiatkowski","sequence":"additional","affiliation":[{"name":"Cepheid, Sunnyvale, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Persing","sequence":"additional","affiliation":[{"name":"Cepheid, Sunnyvale, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Connell","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Alland","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Division of Infectious Diseases, New Jersey Medical School, Rutgers Biomedical and Health Sciences University, Newark, New Jersey, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Bioterrorism: a brief history","author":"Federal Select Agent Program","year":"2017","unstructured":"Federal Select Agent Program. 2017. 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