{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:10:07Z","timestamp":1790799007500,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Campylobacter<\/jats:italic>\n            enteritis is a food-borne or waterborne illness caused almost exclusively by\n            <jats:italic>Campylobacter jejuni<\/jats:italic>\n            and, to a lesser extent, by\n            <jats:italic>Campylobacter coli<\/jats:italic>\n            . These organisms produce indistinguishable clinical diseases and together represent the second most common cause of bacterial diarrhea in the United States and the leading cause of enteric infection throughout the world. The conventional approach to the laboratory diagnosis of\n            <jats:italic>Campylobacter<\/jats:italic>\n            enteritis is based on the recovery of the organism from a stool specimen, which requires the use of a specialized medium incubated at 42\u00b0C for several days in an artificially created microaerophilic environment. Recently, several commercially available enzyme immunoassays (EIAs) have been developed for the direct detection of\n            <jats:italic>C. jejuni<\/jats:italic>\n            and\n            <jats:italic>C. coli<\/jats:italic>\n            in stool specimens. This study compared conventional culture with three EIA methods, the Premier CAMPY EIA (Meridian Bioscience, Cincinnati, OH), the ProSpecT Campylobacter EIA (Remel, Lenexa, KS), and the ImmunoCard STAT! CAMPY test (Meridian Bioscience, Cincinnati, OH), for the detection of\n            <jats:italic>C. jejuni<\/jats:italic>\n            and\n            <jats:italic>C. coli<\/jats:italic>\n            in 485 patient stool samples. Discordant results were arbitrated by using an in-house, real-time PCR assay that was developed and validated by a public health reference laboratory. Following analyses of the discrepant specimens by PCR, the sensitivity and specificity of both the Premier CAMPY and ProSpecT Campylobacter EIAs were 99.3% and 98%, respectively, while the ImmunoCard STAT! CAMPY test had a sensitivity of 98.5% and a specificity of 98.2%. By use of the PCR test as the reference standard, culture detected 127 of 135\n            <jats:italic>Campylobacter<\/jats:italic>\n            -positive stool specimens, yielding a sensitivity of 94.1%. These results showed that the three EIAs evaluated in this study provide a rapid and reliable alternative for the laboratory diagnosis of enteric infections with\n            <jats:italic>C. jejuni<\/jats:italic>\n            and\n            <jats:italic>C. coli<\/jats:italic>\n            and that conventional culture may no longer be recognized as the \u201cgold standard\u201d for diagnosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.00486-10","type":"journal-article","created":{"date-parts":[[2010,9,2]],"date-time":"2010-09-02T02:49:35Z","timestamp":1283395775000},"page":"4022-4027","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Comparison of Premier CAMPY Enzyme Immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY Tests with Culture for Laboratory Diagnosis of\n            <i>Campylobacter<\/i>\n            Enteric Infections"],"prefix":"10.1128","volume":"48","author":[{"given":"Paul A.","family":"Granato","sequence":"first","affiliation":[{"name":"SUNY Upstate Medical University, Syracuse"},{"name":"Laboratory Alliance of Central New York, Liverpool"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory Alliance of Central New York, Liverpool"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Holiday","sequence":"additional","affiliation":[{"name":"Laboratory Alliance of Central New York, Liverpool"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell A.","family":"Rawling","sequence":"additional","affiliation":[{"name":"Laboratory Alliance of Central New York, Liverpool"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Novak-Weekley","sequence":"additional","affiliation":[{"name":"Kaiser Permanente Regional Reference Laboratories, North Hollywood, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tammy","family":"Quinlan","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberlee A.","family":"Musser","sequence":"additional","affiliation":[{"name":"Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"9","volume":"44","year":"2008","unstructured":"Adedayo, O., and B. 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