{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T10:39:36Z","timestamp":1756809576070},"reference-count":35,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Prompted by reports challenging the validity of the low-positive\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            and\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            results generated by the APTIMA Combo 2 assay (Gen-Probe, Incorporated) and by a Centers for Disease Control and Prevention recommendation to confirm\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            - or\n            <jats:italic>C. trachomatis<\/jats:italic>\n            -positive screens by using an alternative amplification target, we report on a comparison of this means of confirmation with an in-house algorithm of repeat testing. Primary clinical specimens yielding\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            - or\n            <jats:italic>C. trachomatis<\/jats:italic>\n            -specific luminescent values between 100,000 and 1,000,000 were repeat tested in duplicate. A subset of specimens was forwarded for confirmatory assays (Gen-Probe) individualized for alternative\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            or\n            <jats:italic>C. trachomatis<\/jats:italic>\n            targets. An 18-month audit revealed that 230 of 29,977\n            <jats:italic>C. trachomatis<\/jats:italic>\n            screens (0.8%) and 41 of 29,064\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            assays (0.1%) yielded low-positive data. When a subset of 40 low-positive\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            screens was repeat tested, 20 (50.0%) remained positive; 22 (55.0%) of the screens remained positive following performance of the confirmatory assay. In contrast, repeat testing of 153 low-positive\n            <jats:italic>C. trachomatis<\/jats:italic>\n            screens yielded a positive result for fewer specimens (\n            <jats:italic>n<\/jats:italic>\n            = 97; 63.4%) than when commercial confirmatory testing was used (\n            <jats:italic>n<\/jats:italic>\n            = 124; 81.0%). However, confirmation of the results for additional\n            <jats:italic>C. trachomatis<\/jats:italic>\n            screens by use of an alternative target did not translate into significant differences in the calculated overall\n            <jats:italic>C. trachomatis<\/jats:italic>\n            -positive screen rates (7.39% by repeat testing versus 7.52% by the confirmatory assay;\n            <jats:italic>P<\/jats:italic>\n            = 0.53). Furthermore, use of the confirmatory assay raised the positive predictive value only 1.8% over that of repeat testing. Molecular confirmatory testing did not significantly enhance the reliability of\n            <jats:italic>C. trachomatis<\/jats:italic>\n            - or\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            -specific nucleic acid amplification testing in this metropolitan setting compared to the reliability of repeat testing.\n          <\/jats:p>","DOI":"10.1128\/jcm.00491-07","type":"journal-article","created":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T01:44:31Z","timestamp":1182390271000},"page":"2793-2797","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of Gen-Probe APTIMA-Based\n            <i>Neisseria gonorrhoeae<\/i>\n            and\n            <i>Chlamydia trachomatis<\/i>\n            Confirmatory Testing in a Metropolitan Setting of High Disease Prevalence"],"prefix":"10.1128","volume":"45","author":[{"given":"Erik","family":"Munson","sequence":"first","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"},{"name":"College of Health Sciences, University of Wisconsin\u2014Milwaukee, Milwaukee, Wisconsin 53201"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivian","family":"Boyd","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolanta","family":"Czarnecka","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Griep","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Lund","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Schaal","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne E.","family":"Hryciuk","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.11.3023-3027.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3089-3093.2004"},{"key":"e_1_3_2_4_2","first-page":"1","volume":"51","year":"2002","unstructured":"Centers for Disease Control and Prevention. 2002. 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