{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:21:23Z","timestamp":1792736483458,"version":"4.5.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The incidence of antimicrobial-resistant\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay for surveillance use to detect mutations in genes associated with decreased susceptibility to cephalosporins that can be applied to both culture isolates and clinical samples. Real-time PCR assays were developed to detect single nucleotide polymorphisms (SNPs) in\n            <jats:italic>ponA<\/jats:italic>\n            ,\n            <jats:italic>mtrR<\/jats:italic>\n            ,\n            <jats:italic>penA<\/jats:italic>\n            ,\n            <jats:italic>porB<\/jats:italic>\n            , and one\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -specific marker (\n            <jats:italic>porA<\/jats:italic>\n            ). We tested the real-time PCR assay with 252 gonococcal isolates, 50 nongonococcal isolates, 24\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -negative NAAT specimens, and 34\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -positive NAAT specimens. Twenty-four of the\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -positive NAAT specimens had matched culture isolates. Assay results were confirmed by comparison with whole-genome sequencing data. For 252\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            strains, the agreement between the DNA sequence and real-time PCR was 100% for\n            <jats:italic>porA<\/jats:italic>\n            ,\n            <jats:italic>ponA<\/jats:italic>\n            , and\n            <jats:italic>penA<\/jats:italic>\n            , 99.6% for\n            <jats:italic>mtrR<\/jats:italic>\n            , and 95.2% for\n            <jats:italic>porB<\/jats:italic>\n            . The presence of \u22652 SNPs correlated with decreased susceptibility to ceftriaxone (sensitivities of &gt;98%) and cefixime (sensitivities of &gt;96%). Of 24 NAAT specimens with matched cultures, the agreement between the DNA sequence and real-time PCR was 100% for\n            <jats:italic>porB<\/jats:italic>\n            , 95.8% for\n            <jats:italic>ponA<\/jats:italic>\n            and\n            <jats:italic>mtrR<\/jats:italic>\n            , and 91.7% for\n            <jats:italic>penA<\/jats:italic>\n            . We demonstrated the utility of a real-time PCR assay for sensitive detection of known markers for the decreased susceptibility to cephalosporins in\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            . Preliminary results with clinical NAAT specimens were also promising, as they correlated well with bacterial culture results.\n          <\/jats:p>","DOI":"10.1128\/jcm.00493-15","type":"journal-article","created":{"date-parts":[[2015,4,16]],"date-time":"2015-04-16T03:01:30Z","timestamp":1429153290000},"page":"2042-2048","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Molecular Assay for Detection of Genetic Markers Associated with Decreased Susceptibility to Cephalosporins in Neisseria gonorrhoeae"],"prefix":"10.1128","volume":"53","author":[{"given":"S. W.","family":"Peterson","sequence":"first","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Martin","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Demczuk","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Bharat","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Hoang","sequence":"additional","affiliation":[{"name":"British Columbia Centres for Disease Control Public Health Microbiology and Reference Laboratory, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Wylie","sequence":"additional","affiliation":[{"name":"Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Allen","sequence":"additional","affiliation":[{"name":"Public Health Ontario Laboratories, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Ste-Anne-De-Bellevue, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Tyrrell","sequence":"additional","affiliation":[{"name":"Provincial Laboratory for Public Health, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Horsman","sequence":"additional","affiliation":[{"name":"Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Haldane","sequence":"additional","affiliation":[{"name":"Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Garceau","sequence":"additional","affiliation":[{"name":"Dr. G.L. Dumont Hospital, Moncton, New Brunswick, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Wong","sequence":"additional","affiliation":[{"name":"Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. R.","family":"Mulvey","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Notifiable diseases on-line","author":"Public Health Agency of Canada","year":"2014","unstructured":"Public Health Agency of Canada. 2014. Notifiable diseases on-line. 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