{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T17:29:32Z","timestamp":1769016572593,"version":"3.49.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2017,8,23]],"date-time":"2017-08-23T00:00:00Z","timestamp":1503446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100004324","name":"Astellas Pharma Inc.","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004324","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An epidemiological, multicenter, noninterventional, observational case-control study was conducted to describe the performance of serum beta-\n            <jats:sc>d<\/jats:sc>\n            -glucan (BDG) and\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            PCR in blood, serum, and sterile samples for the diagnosis of invasive candidiasis (IC) in very-low-birth-weight (VLBW) preterm neonates and to compare these techniques with culture of samples from blood and other sterile sites. Seventeen centers participated in the study, and the number of episodes analyzed was 159. A total of 9 episodes of IC from 9 patients (7 confirmed and 2 probable) and 150 episodes of suspected sepsis from 117 controls were identified. The prevalence of IC was 5.7% (95% confidence interval [95% CI], 2.1 to 9.3). The mortality was significantly higher in episodes of IC (44.4%) than in the non-IC episodes (11.1%,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01). The sensitivity and specificity of the PCR performed on blood\/serum samples were 87.5% and 81.6%, respectively. The sensitivity and specificity of the BDG results were lower (75.0% and 64.6%). For cases with negative culture results, the PCR and the BDG results were positive in 27 (17.4%) and 52 (33.5%) episodes, respectively. The presence of multiorgan failure, improvement with empirical antifungal therapy, thrombocytopenia, and\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            colonization were significantly associated (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.01) with PCR or BDG positivity regardless of the results of the cultures. Serum BDG analysis and\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            PCR could be used as complementary diagnostic techniques to detect IC in VLBW neonates.\n          <\/jats:p>","DOI":"10.1128\/jcm.00496-17","type":"journal-article","created":{"date-parts":[[2017,6,29]],"date-time":"2017-06-29T00:30:29Z","timestamp":1498696229000},"page":"2752-2764","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Performance of a Quantitative PCR-Based Assay and Beta-\n            <scp>d<\/scp>\n            -Glucan Detection for Diagnosis of Invasive Candidiasis in Very-Low-Birth-Weight Preterm Neonatal Patients (CANDINEO Study)"],"prefix":"10.1128","volume":"55","author":[{"given":"Jose Tomas","family":"Ramos","sequence":"first","affiliation":[{"name":"Hospital Universitario Cl\u00ednico San Carlos, Departamento de Pediatr\u00eda, and Universidad Complutense, Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdISSC), Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"Villar","sequence":"additional","affiliation":[{"name":"Hospital Materno-Infantil Gregorio Mara\u00f1\u00f3n, Servicio de Neonatolog\u00eda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emilio","family":"Bouza","sequence":"additional","affiliation":[{"name":"Hospital General Universitario Gregorio Mara\u00f1\u00f3n CIBERES, IISGM Universidad Complutense de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Bergon-Sendin","sequence":"additional","affiliation":[{"name":"Hospital Universitario 12 de Octubre, Servicio de Neonatolog\u00eda, Universidad Complutense, and Red de Salud Materno Infantil y del Desarrollo SAMID, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Perez Rivilla","sequence":"additional","affiliation":[{"name":"Hospital Universitario 12 de Octubre, Servicio de Microbiolog\u00eda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caridad Tapia","family":"Collados","sequence":"additional","affiliation":[{"name":"Hospital General Universitario de Alicante, Servicio de Neonatolog\u00eda, Alicante, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano","family":"Andreu","sequence":"additional","affiliation":[{"name":"Hospital General Universitario de Alicante, Servicio de Microbiolog\u00eda, Alicante, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Candelaria Santana","family":"Reyes","sequence":"additional","affiliation":[{"name":"Hospital Materno-Infantil Insular de Las Palmas, Servicio de Neonatolog\u00eda, Las Palmas de Gran Canaria, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Isolina","family":"Campos-Herrero","sequence":"additional","affiliation":[{"name":"Hospital Universitario de Gran Canaria Doctor Negr\u00edn, Hospital Materno-Infantil, Servicio de Microbiolog\u00eda, Las Palmas de Gran Canaria, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon L\u00f3pez","family":"de Heredia","sequence":"additional","affiliation":[{"name":"Hospital de Cruces, Servicio de Neonatolog\u00eda, Bilbao, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Cruz L\u00f3pez","family":"Herrera","sequence":"additional","affiliation":[{"name":"Hospital de Cruces, Servicio de Neonatolog\u00eda, Bilbao, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paloma Anguita","family":"Alonso","sequence":"additional","affiliation":[{"name":"Astellas Pharma S.A., Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen Rosa","family":"Pall\u00e1s-Alonso","sequence":"additional","affiliation":[{"name":"Hospital Universitario 12 de Octubre, Servicio de Neonatolog\u00eda, Universidad Complutense, and Red de Salud Materno Infantil y del Desarrollo SAMID, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Cuenca-Estrella","sequence":"additional","affiliation":[{"name":"Instituto de Salud Carlos III, Majadahonda, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"the CANDINEO Study 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