{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:21:29Z","timestamp":1785216089937,"version":"3.55.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n The FTA card (Whatman) was assessed for its utility as a molecular epidemiological tool in collecting samples from patients with leishmaniasis in Peru because the card has a variety of merits; it is less invasive for patients and easy to handle for both physicians and other medical personnel for sample collection or diagnosis, in addition to its simplicity and easy countrywide and\/or intercountry transportation for analysis. Samples were collected from 132 patients suspected of having leishmaniasis, and\n Leishmania<\/jats:italic>\n species were successfully identified in samples from 81 patients in 15 departments of Peru by cytochrome\n b<\/jats:italic>\n and mannose phosphate isomerase gene analyses. Of these, 61.7% were identified as\n Leishmania<\/jats:italic>\n (\n Viannia<\/jats:italic>\n )\n peruviana<\/jats:italic>\n , 22.2% as\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n braziliensis<\/jats:italic>\n , 12.3% as\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n guyanensis<\/jats:italic>\n , 2.5% as\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n shawi<\/jats:italic>\n , and 1.2% as\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n lainsoni<\/jats:italic>\n . The three predominant species,\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n peruviana<\/jats:italic>\n ,\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n braziliensis<\/jats:italic>\n , and\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n guyanensis<\/jats:italic>\n , were mainly found in the Andean highlands, in the tropical rainforest, and in northern and central rainforest regions, respectively. This is the first time\n L.<\/jats:italic>\n (\n V.<\/jats:italic>\n )\n shawi<\/jats:italic>\n has been identified outside Brazil. The present study showed that the FTA card will be a useful tool for the ecological study of different forms of leishmaniasis. Furthermore, collecting samples directly from patients' lesions by using the FTA card eliminates (i) the possibility of contamination of\n Leishmania<\/jats:italic>\n isolates during short- and\/or long-term passages of culture\n in vitro<\/jats:italic>\n in each laboratory and (ii) pain and suffering of patients from taking samples by skin biopsy.\n <\/jats:p>","DOI":"10.1128\/jcm.00498-10","type":"journal-article","created":{"date-parts":[[2010,8,19]],"date-time":"2010-08-19T02:15:13Z","timestamp":1282184113000},"page":"3661-3665","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Use of FTA Cards for Direct Sampling of Patients' Lesions in the Ecological Study of Cutaneous Leishmaniasis"],"prefix":"10.1128","volume":"48","author":[{"given":"Hirotomo","family":"Kato","sequence":"first","affiliation":[{"name":"Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham G.","family":"Ca\u0301ceres","sequence":"additional","affiliation":[{"name":"Seccio\u0301n de Entomologi\u0301a, Instituto de Medicina Tropical Daniel A. Carrion, Facultad de Medicina, Universidad Nacional Mayor de San Marcos"},{"name":"Laboratorio de Entomologi\u0301a, Instituto Nacional de Salud, Lima, Peru\u0301"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuyuki","family":"Mimori","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Health Sciences, Kumamoto University, Kumamoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Ishimaru","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal S. M.","family":"Sayed","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan"},{"name":"Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megumi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Uezato","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenin N.","family":"Velez","sequence":"additional","affiliation":[{"name":"Departamento de Leishmaniasis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo A. L.","family":"Gomez","sequence":"additional","affiliation":[{"name":"Departamento de Oncocercosis, Servicio Nacional de Erradicacion de la Malaria (SNEM), Ministerio de Salud Publica, Guayquil, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Hashiguchi","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/518041"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/648719"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2009.02361.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.296"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/592509"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2004.03.004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90056-8"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02555-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.79.719"},{"key":"e_1_3_2_11_2","first-page":"1","year":"1987","unstructured":"Lainson, R., and J. 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