{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:04:09Z","timestamp":1791216249204,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The differentiation of species within the\n            <jats:named-content content-type=\"genus-species\">Streptococcus mitis<\/jats:named-content>\n            group has posed a problem in the routine diagnostic microbiology laboratory for some time. It also constitutes a major weakness of recently introduced matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) fingerprinting systems. As the phylogenetic resolution of the spectral similarity measures employed by these systems is insufficient to reliably distinguish between the most closely related members of the group, the major pathogen\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            is frequently misidentified. In this study, a comparative analysis of MALDI-TOF spectra of several species from the\n            <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n            group has been performed in order to identify single peaks that could be used to improve mass spectrometry-based identification of the respective species. A characteristic peak profile could be identified that unambiguously distinguished the 14\n            <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n            isolates studied from 33 nonpneumococcal isolates of the\n            <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n            group. In addition, specific peak combinations could be assigned to other members of the group. The findings of this study suggest that it is possible to distinguish different species of the\n            <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n            group by close analysis of their mass peak profiles.\n          <\/jats:p>","DOI":"10.1128\/jcm.00508-12","type":"journal-article","created":{"date-parts":[[2012,6,21]],"date-time":"2012-06-21T13:33:52Z","timestamp":1340285632000},"page":"2863-2867","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":92,"title":["Differentiation of Streptococcus pneumoniae from Nonpneumococcal Streptococci of the Streptococcus mitis Group by Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry"],"prefix":"10.1128","volume":"50","author":[{"given":"Anja M.","family":"Werno","sequence":"first","affiliation":[{"name":"Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand"},{"name":"Department of Pathology, University of Otago, Christchurch, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Christner","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, Virology and Hygiene, University Medical Centre Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor P.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Murdoch","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Canterbury Health Laboratories, Christchurch, New Zealand"},{"name":"Department of Pathology, University of Otago, Christchurch, New 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