{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T21:10:47Z","timestamp":1792271447012,"version":"4.4.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Antigen-based immunoassays are currently needed for point-of-care quantification of\n            <jats:italic>Loa loa<\/jats:italic>\n            microfilariae (mf). Coupling transcriptomic approaches with bioinformatic analysis, we have identified 11 specific putative proteins (coding mRNAs) with potential utility as biomarkers of patent (mf\n            <jats:sup>\n              <jats:bold>+<\/jats:bold>\n            <\/jats:sup>\n            )\n            <jats:italic>L. loa<\/jats:italic>\n            infection. We successfully developed antigen capture immunoassays to quantify 2 (LOAG_14221 and LOAG_15846) of these proteins in individual plasma\/serum samples. Of the 2 quantifiable circulating biomarkers, LOAG_14221 showed the highest degree of specificity, particularly with a monoclonal antibody-based immunoassay. Moreover, the levels of LOAG_14221 in\n            <jats:italic>L. loa<\/jats:italic>\n            mf\n            <jats:sup>\n              <jats:bold>+<\/jats:bold>\n            <\/jats:sup>\n            patients were positively correlated to the mf densities in the corresponding blood samples (\n            <jats:italic>r<\/jats:italic>\n            = 0.53 and\n            <jats:italic>P<\/jats:italic>\n            = 0.008 for polyclonal assay;\n            <jats:italic>r<\/jats:italic>\n            = 0.54 and\n            <jats:italic>P<\/jats:italic>\n            = 0.004 for monoclonal assay). Thus, LOAG_14221 is a very promising biomarker that will be exploited in a quantitative point-of-care immunoassay for determination of\n            <jats:italic>L. loa<\/jats:italic>\n            mf densities.\n          <\/jats:p>","DOI":"10.1128\/jcm.00513-17","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:40:27Z","timestamp":1498092027000},"page":"2671-2678","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Discovery of Specific Antigens That Can Predict Microfilarial Intensity in\n            <i>Loa loa<\/i>\n            Infection"],"prefix":"10.1128","volume":"55","author":[{"given":"Papa M.","family":"Drame","sequence":"first","affiliation":[{"name":"Helminth Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasisekhar","family":"Bennuru","sequence":"additional","affiliation":[{"name":"Helminth Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas B.","family":"Nutman","sequence":"additional","affiliation":[{"name":"Helminth Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001210"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70263-9"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)11094-1"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000007423"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2011.10.003"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)30405-4"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.2000.00318.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2008.12.016"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00525-14"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0746"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001299"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa3480"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02132-15"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1145406"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.216"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2585"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-788"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02028-16"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGENETICS.112.962977"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.07.007"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.72-75.1995"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2014.02.031"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2015.05.008"},{"key":"e_1_3_3_25_2","first-page":"745","article-title":"Two-site sandwich ELISA for detection of Plasmodium vivax blood stage antigens using monoclonal and polyclonal antibodies","volume":"23","author":"Khusmith S","year":"1992","unstructured":"Khusmith S Intapan P Tharavanij S Tuntrakul S Indravijit KA Bunnag D . 1992. 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