{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:19:32Z","timestamp":1790716772437,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["10-IAHU-03"],"award-info":[{"award-number":["10-IAHU-03"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>\n            We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the detection and culture of\n            <jats:italic>Treponema<\/jats:italic>\n            species from the human oral cavity. Of the 44 oral samples cultivated, 15 (34%) were found to be positive for\n            <jats:italic>Treponema<\/jats:italic>\n            using electron microscopy and were also culture positive. All were subcultured on agar plates; based on genome sequencing and analyses, 10 were strains of\n            <jats:named-content content-type=\"genus-species\">Treponema pectinovorum<\/jats:named-content>\n            and 5 were strains of\n            <jats:named-content content-type=\"genus-species\">Treponema denticola<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.00517-19","type":"journal-article","created":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T10:50:45Z","timestamp":1563879045000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry for\n            <i>Treponema<\/i>\n            Culture and Identification from the Oral Cavity"],"prefix":"10.1128","volume":"57","author":[{"given":"Souad","family":"Belkacemi","sequence":"first","affiliation":[{"name":"Microbes Evolution Phylogeny, and Infections, Aix-Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-Hopitaux de Marseille, Marseille, France"},{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Bou Khalil","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny, and Infections, Aix-Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-Hopitaux de Marseille, Marseille, France"},{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Ominami","sequence":"additional","affiliation":[{"name":"Science and Medical Systems Business Group, Hitachi High-Technologies Corp., Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Hisada","sequence":"additional","affiliation":[{"name":"Research and Development Group, Hitachi, Ltd., Saitama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Fontanini","sequence":"additional","affiliation":[{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurelia","family":"Caputo","sequence":"additional","affiliation":[{"name":"H\u00f4pital de la Timone, Assistance Publique-Hopitaux de Marseille, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Levasseur","sequence":"additional","affiliation":[{"name":"UMR VITROME, Aix Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, SSA, Assistance Publique-Hopitaux de Marseille, IHU-M\u00e9diterran\u00e9e Infection, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Lagier","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny, and Infections, Aix-Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-Hopitaux de Marseille, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saber","family":"Khelaifia","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny, and Infections, Aix-Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-Hopitaux de Marseille, Marseille, France"},{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny, and Infections, Aix-Marseille Universit\u00e9, Institut de Recherche pour le D\u00e9veloppement, Assistance Publique-Hopitaux de Marseille, Marseille, France"},{"name":"Institut Hospitalo-Universitaire M\u00e9diterran\u00e9e Infection, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-21609-6_15"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30746-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1909.00050160040002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-41882-y"},{"key":"e_1_3_3_6_2","first-page":"43","article-title":"Identification of four Treponema species in subgingival samples by nested-PCR and their correlation with clinical diagnosis","volume":"71","author":"Dabu B","year":"2012","unstructured":"Dabu B, Mironiuc-Cureu M, Jardan D, Szmal C, Dumitriu S. 2012. 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