{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T03:37:36Z","timestamp":1789529856027,"version":"build-2803163510"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Azole-resistant\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            isolates in our institution, a Belgian university hospital, we conducted a laboratory-based surveillance between June 2015 and October 2016. Two different approaches were used: a prospective culture-based surveillance using VIPcheck on unselected\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 109 patients, including 19 patients with proven or probable invasive aspergillosis [IA]), followed by molecular detection of mutations conferring azole resistance, and a retrospective detection of azole-resistant\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            in bronchoalveolar lavage fluid using the commercially available AsperGenius PCR (\n            <jats:italic>n<\/jats:italic>\n            = 100 patients, including 29 patients with proven or probable IA). By VIPcheck, 25 azole-resistant\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            specimens were isolated from 14 patients (12.8%). Of these 14 patients, only 2 had proven or probable IA (10.5%). Mutations at the\n            <jats:italic>cyp51A<\/jats:italic>\n            gene were observed in 23 of the 25\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            isolates; TR\n            <jats:sub>34<\/jats:sub>\n            \/L98H was the most prevalent mutation (46.7%), followed by TR\n            <jats:sub>46<\/jats:sub>\n            \/Y121F\/T289A (26.7%). Twenty-seven (27%) patients were positive for the presence of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species by AsperGenius PCR.\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            was detected by AsperGenius in 20 patients, and 3 of these patients carried\n            <jats:italic>cyp51A<\/jats:italic>\n            mutations. Two patients had proven or probable IA and\n            <jats:italic>cyp51A<\/jats:italic>\n            mutation (11.7%). Our study has shown that the detection of azole-resistant\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            in clinical isolates was a frequent finding in our institution. Hence, a rapid method for resistance detection may be useful to improve patient management. Centers that care for immunocompromised patients should perform routine surveillance to determine their local epidemiology.\n          <\/jats:p>","DOI":"10.1128\/jcm.00520-17","type":"journal-article","created":{"date-parts":[[2017,5,18]],"date-time":"2017-05-18T00:40:32Z","timestamp":1495068032000},"page":"2391-2399","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Culture-Based Methods and Molecular Tools for Azole-Resistant Aspergillus fumigatus Detection in a Belgian University Hospital"],"prefix":"10.1128","volume":"55","author":[{"given":"I.","family":"Montesinos","sequence":"first","affiliation":[{"name":"Department of Microbiology, CUB-Erasme, Universit\u00e9 Libre de Bruxelles, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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